TY - JOUR
T1 - Growing and analyzing biofilms in flow cells.
AU - Sternberg, Claus
AU - Tolker-Nielsen, Tim
N1 - Keywords: Biofilms; Bioreactors; Microscopy, Confocal
PY - 2006
Y1 - 2006
N2 - The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. The biofilms in flow cells develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow cell-grown biofilms are addressed.
AB - The setup of flow cell systems for the study of microbial biofilms and methods for the analysis of structural biofilm development are described in this unit. Use of flow cells allows direct microscopic investigation of biofilm development. The biofilms in flow cells develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow cell and the bubble trap, assembly and sterilization of the flow cell system, inoculation of the flow cells, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow cell-grown biofilms are addressed.
U2 - 10.1002/9780471729259.mc01b02s00
DO - 10.1002/9780471729259.mc01b02s00
M3 - Journal article
C2 - 18770573
SN - 1934-8525
VL - Chapter 1
SP - Unit 1B.2
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
ER -