Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats

C Orskov; Steen Seier Poulsen

Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats

Endocrinology and Metabolism

62 Citationer (Scopus)

Abstract

We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.

Originalsprog	Engelsk
Tidsskrift	Diabetes
Vol/bind	40
Udgave nummer	10
Sider (fra-til)	1292-6
Antal sider	5
ISSN	0012-1797
Status	Udgivet - okt. 1991

Citationsformater

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title = "Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats",

abstract = "We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.",

keywords = "Animals, Autoradiography, Female, Glucagon, Glucagon-Like Peptide 1, Glucagon-Like Peptides, Iodine Radioisotopes, Islets of Langerhans, Male, Peptide Fragments, Peptides, Radioligand Assay, Rats, Rats, Inbred Strains, Receptors, Cell Surface, Receptors, Glucagon, Stomach",

author = "C Orskov and Poulsen, {Steen Seier}",

year = "1991",

month = oct,

language = "English",

volume = "40",

pages = "1292--6",

journal = "Diabetes",

issn = "0012-1797",

publisher = "American Diabetes Association",

number = "10",

}

TY - JOUR

T1 - Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats

AU - Orskov, C

AU - Poulsen, Steen Seier

PY - 1991/10

Y1 - 1991/10

N2 - We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.

AB - We demonstrated specific binding of the insulin-releasing hormone glucagonlike peptide (GLP)-I-(7-36)-amide, an intestinal product of proglucagon, to pancreatic islet cells by autoradiography using 125I-labeled GLP-I-(7-36)-amide incubated with tissue specimens of extracerebral rat organs. We also found binding of 125I-GLP-I to insulin-, glucagon-, and somatostatin-immunoreactive cells by combined autoradiographic and immunohistochemical analysis of pancreatic specimens using antisera against insulin, glucagon, and somatostatin. An accumulation of radioactivity was also observed in the stomach surface epithelium but could not be prevented by excess unlabeled peptide. No binding was found in other tissues investigated, including the lungs and the small intestinal mucosa. Localization of the binding sites identifies the pancreatic islets as the prime target for GLP-I-(7-36)-amide and suggests that it exerts direct effects on islet cells.

KW - Animals

KW - Autoradiography

KW - Female

KW - Glucagon

KW - Glucagon-Like Peptide 1

KW - Glucagon-Like Peptides

KW - Iodine Radioisotopes

KW - Islets of Langerhans

KW - Male

KW - Peptide Fragments

KW - Peptides

KW - Radioligand Assay

KW - Rats

KW - Rats, Inbred Strains

KW - Receptors, Cell Surface

KW - Receptors, Glucagon

KW - Stomach

M3 - Journal article

C2 - 1657666

SN - 0012-1797

VL - 40

SP - 1292

EP - 1296

JO - Diabetes

JF - Diabetes

IS - 10

ER -

Glucagonlike peptide-I-(7-36)-amide receptors only in islets of Langerhans. Autoradiographic survey of extracerebral tissues in rats

Abstract

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