Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

Heinrich Dircksen; Susanne Neupert; Reinhard Predel; Peter Verleyen; Jurgen Huybrechts; Johannes Strauss; Frank Hauser; Elisabeth Stafflinger; Martina Schneider; Kevin Pauwels; Liliane Schoofs; Cornelis J P Grimmelikhuijzen

doi:10.1021/pr200284e

Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

Heinrich Dircksen, Susanne Neupert, Reinhard Predel, Peter Verleyen, Jurgen Huybrechts, Johannes Strauss, Frank Hauser, Elisabeth Stafflinger, Martina Schneider, Kevin Pauwels, Liliane Schoofs, Cornelis J P Grimmelikhuijzen

Cell- and Neurobiology

142 Citationer (Scopus)

Abstract

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

Originalsprog	Engelsk
Tidsskrift	Journal of Proteome Research
Vol/bind	10
Udgave nummer	10
Sider (fra-til)	4478-4504
Antal sider	27
ISSN	1535-3893
DOI	https://doi.org/10.1021/pr200284e
Status	Udgivet - 7 okt. 2011

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10.1021/pr200284e

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title = "Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones",

abstract = "We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.",

keywords = "Adipokines, Amino Acid Sequence, Animals, Computational Biology, Daphnia, Expressed Sequence Tags, Female, Genomics, Mass Spectrometry, Molecular Sequence Data, Neuropeptides, Peptides, Proteins, Proteomics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transcriptome",

author = "Heinrich Dircksen and Susanne Neupert and Reinhard Predel and Peter Verleyen and Jurgen Huybrechts and Johannes Strauss and Frank Hauser and Elisabeth Stafflinger and Martina Schneider and Kevin Pauwels and Liliane Schoofs and Grimmelikhuijzen, {Cornelis J P}",

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doi = "10.1021/pr200284e",

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volume = "10",

pages = "4478--4504",

journal = "Journal of Proteome Research",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

AU - Dircksen, Heinrich

AU - Neupert, Susanne

AU - Predel, Reinhard

AU - Verleyen, Peter

AU - Huybrechts, Jurgen

AU - Strauss, Johannes

AU - Hauser, Frank

AU - Stafflinger, Elisabeth

AU - Schneider, Martina

AU - Pauwels, Kevin

AU - Schoofs, Liliane

AU - Grimmelikhuijzen, Cornelis J P

PY - 2011/10/7

Y1 - 2011/10/7

N2 - We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

AB - We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

KW - Adipokines

KW - Amino Acid Sequence

KW - Animals

KW - Computational Biology

KW - Daphnia

KW - Expressed Sequence Tags

KW - Female

KW - Genomics

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Neuropeptides

KW - Peptides

KW - Proteins

KW - Proteomics

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Homology, Amino Acid

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Transcriptome

U2 - 10.1021/pr200284e

DO - 10.1021/pr200284e

M3 - Journal article

C2 - 21830762

SN - 1535-3893

VL - 10

SP - 4478

EP - 4504

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 10

ER -

Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

Abstract

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