Genomics and peptidomics of neuropeptides and protein hormones present in the parasitic wasp Nasonia vitripennis

Frank Hauser; Susanne Neupert; Michael Williamson; Reinhard Predel; Yoshiaki Tanaka; Cornelis J P Grimmelikhuijzen

doi:10.1021/pr100570j

Genomics and peptidomics of neuropeptides and protein hormones present in the parasitic wasp Nasonia vitripennis

Frank Hauser, Susanne Neupert, Michael Williamson, Reinhard Predel, Yoshiaki Tanaka, Cornelis J P Grimmelikhuijzen

Cell- and Neurobiology

126 Citationer (Scopus)

Abstract

Neuropeptides and protein hormones constitute a very important group of signaling molecules, regulating central physiological processes such as reproduction, development, and behavior. Using a bioinformatics approach, we screened the recently sequenced genome of the parasitic wasp, Nasonia vitripennis, for the presence of these signaling molecules and annotated 30 precursor genes encoding 51 different mature neuropeptides or protein hormones. Twenty-four of the predicted mature Nasonia neuropeptides could be experimentally confirmed by mass spectrometry. We also discovered a completely novel neuropeptide gene in Nasonia, coding for peptides containing the C-terminal sequence RYamide. This gene has orthologs in nearly all arthropods with a sequenced genome, and its expression in mosquitoes was confirmed by mass spectrometry. No precursor could be identified for N-terminally extended FMRFamides, even though their putative G protein coupled receptor (GPCR) is present in the Nasonia genome. Neither the precursor nor the putative receptor could be identified for allatostatin-B, capa, the glycoprotein hormones GPA2/GPB5, kinin, proctolin, sex peptide, and sulfakinin, arguing that these signaling systems are truly absent in the wasp. Also, antidiuretic factors, allatotropin, and NPLP-like precursors are missing in Nasonia, but here the receptors have not been identified in any insect, so far. Nasonia (Hymenoptera) has the lowest number of neuropeptide precursor genes compared to Drosophila melanogaster, Aedes aegypti (both Diptera), Bombyx mori (Lepidoptera), Tribolium castaneum (Coleoptera), Apis mellifera (Hymenoptera), and Acyrthosiphon pisum (Hemiptera). This lower number of neuropeptide genes might be related to Nasonia's parasitic life.

Originalsprog	Engelsk
Tidsskrift	Journal of Proteome Research
Vol/bind	9
Udgave nummer	10
Sider (fra-til)	5296-310
Antal sider	15
ISSN	1535-3893
DOI	https://doi.org/10.1021/pr100570j
Status	Udgivet - 1 okt. 2010

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10.1021/pr100570j

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title = "Genomics and peptidomics of neuropeptides and protein hormones present in the parasitic wasp Nasonia vitripennis",

abstract = "Neuropeptides and protein hormones constitute a very important group of signaling molecules, regulating central physiological processes such as reproduction, development, and behavior. Using a bioinformatics approach, we screened the recently sequenced genome of the parasitic wasp, Nasonia vitripennis, for the presence of these signaling molecules and annotated 30 precursor genes encoding 51 different mature neuropeptides or protein hormones. Twenty-four of the predicted mature Nasonia neuropeptides could be experimentally confirmed by mass spectrometry. We also discovered a completely novel neuropeptide gene in Nasonia, coding for peptides containing the C-terminal sequence RYamide. This gene has orthologs in nearly all arthropods with a sequenced genome, and its expression in mosquitoes was confirmed by mass spectrometry. No precursor could be identified for N-terminally extended FMRFamides, even though their putative G protein coupled receptor (GPCR) is present in the Nasonia genome. Neither the precursor nor the putative receptor could be identified for allatostatin-B, capa, the glycoprotein hormones GPA2/GPB5, kinin, proctolin, sex peptide, and sulfakinin, arguing that these signaling systems are truly absent in the wasp. Also, antidiuretic factors, allatotropin, and NPLP-like precursors are missing in Nasonia, but here the receptors have not been identified in any insect, so far. Nasonia (Hymenoptera) has the lowest number of neuropeptide precursor genes compared to Drosophila melanogaster, Aedes aegypti (both Diptera), Bombyx mori (Lepidoptera), Tribolium castaneum (Coleoptera), Apis mellifera (Hymenoptera), and Acyrthosiphon pisum (Hemiptera). This lower number of neuropeptide genes might be related to Nasonia's parasitic life.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Genomics, Insect Proteins, Insects, Molecular Sequence Data, Neuropeptides, Neurotransmitter Agents, Proteomics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Wasps",

author = "Frank Hauser and Susanne Neupert and Michael Williamson and Reinhard Predel and Yoshiaki Tanaka and Grimmelikhuijzen, {Cornelis J P}",

year = "2010",

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doi = "10.1021/pr100570j",

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journal = "Journal of Proteome Research",

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TY - JOUR

T1 - Genomics and peptidomics of neuropeptides and protein hormones present in the parasitic wasp Nasonia vitripennis

AU - Hauser, Frank

AU - Neupert, Susanne

AU - Williamson, Michael

AU - Predel, Reinhard

AU - Tanaka, Yoshiaki

AU - Grimmelikhuijzen, Cornelis J P

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Neuropeptides and protein hormones constitute a very important group of signaling molecules, regulating central physiological processes such as reproduction, development, and behavior. Using a bioinformatics approach, we screened the recently sequenced genome of the parasitic wasp, Nasonia vitripennis, for the presence of these signaling molecules and annotated 30 precursor genes encoding 51 different mature neuropeptides or protein hormones. Twenty-four of the predicted mature Nasonia neuropeptides could be experimentally confirmed by mass spectrometry. We also discovered a completely novel neuropeptide gene in Nasonia, coding for peptides containing the C-terminal sequence RYamide. This gene has orthologs in nearly all arthropods with a sequenced genome, and its expression in mosquitoes was confirmed by mass spectrometry. No precursor could be identified for N-terminally extended FMRFamides, even though their putative G protein coupled receptor (GPCR) is present in the Nasonia genome. Neither the precursor nor the putative receptor could be identified for allatostatin-B, capa, the glycoprotein hormones GPA2/GPB5, kinin, proctolin, sex peptide, and sulfakinin, arguing that these signaling systems are truly absent in the wasp. Also, antidiuretic factors, allatotropin, and NPLP-like precursors are missing in Nasonia, but here the receptors have not been identified in any insect, so far. Nasonia (Hymenoptera) has the lowest number of neuropeptide precursor genes compared to Drosophila melanogaster, Aedes aegypti (both Diptera), Bombyx mori (Lepidoptera), Tribolium castaneum (Coleoptera), Apis mellifera (Hymenoptera), and Acyrthosiphon pisum (Hemiptera). This lower number of neuropeptide genes might be related to Nasonia's parasitic life.

AB - Neuropeptides and protein hormones constitute a very important group of signaling molecules, regulating central physiological processes such as reproduction, development, and behavior. Using a bioinformatics approach, we screened the recently sequenced genome of the parasitic wasp, Nasonia vitripennis, for the presence of these signaling molecules and annotated 30 precursor genes encoding 51 different mature neuropeptides or protein hormones. Twenty-four of the predicted mature Nasonia neuropeptides could be experimentally confirmed by mass spectrometry. We also discovered a completely novel neuropeptide gene in Nasonia, coding for peptides containing the C-terminal sequence RYamide. This gene has orthologs in nearly all arthropods with a sequenced genome, and its expression in mosquitoes was confirmed by mass spectrometry. No precursor could be identified for N-terminally extended FMRFamides, even though their putative G protein coupled receptor (GPCR) is present in the Nasonia genome. Neither the precursor nor the putative receptor could be identified for allatostatin-B, capa, the glycoprotein hormones GPA2/GPB5, kinin, proctolin, sex peptide, and sulfakinin, arguing that these signaling systems are truly absent in the wasp. Also, antidiuretic factors, allatotropin, and NPLP-like precursors are missing in Nasonia, but here the receptors have not been identified in any insect, so far. Nasonia (Hymenoptera) has the lowest number of neuropeptide precursor genes compared to Drosophila melanogaster, Aedes aegypti (both Diptera), Bombyx mori (Lepidoptera), Tribolium castaneum (Coleoptera), Apis mellifera (Hymenoptera), and Acyrthosiphon pisum (Hemiptera). This lower number of neuropeptide genes might be related to Nasonia's parasitic life.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Genomics

KW - Insect Proteins

KW - Insects

KW - Molecular Sequence Data

KW - Neuropeptides

KW - Neurotransmitter Agents

KW - Proteomics

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Homology, Amino Acid

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Wasps

U2 - 10.1021/pr100570j

DO - 10.1021/pr100570j

M3 - Journal article

C2 - 20695486

SN - 1535-3893

VL - 9

SP - 5296

EP - 5310

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 10

ER -

Genomics and peptidomics of neuropeptides and protein hormones present in the parasitic wasp Nasonia vitripennis

Abstract

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