Genomic and Evolutionary Perspectives on Sulfur Metabolism in Green Sulfur Bacteria

TY - CHAP

T1 - Genomic and Evolutionary Perspectives on Sulfur Metabolism in Green Sulfur Bacteria

AU - Frigaard, Niels-Ulrik

AU - Bryant, Donald A.

PY - 2008

Y1 - 2008

N2 - Green sulfur bacteria (GSB) are anaerobic photoautotrophs that oxidize sulfide, elemental sulfur, thiosulfate, ferrous iron, and hydrogen for growth. We present here an analysis of the distribution and evolution of enzymes involved in oxidation of sulfur compounds in GSB based on genome sequence data from 12 strains. Sulfide:quinone reductase (SQR) is found in all strains. Chlorobium ferrooxidans, which cannot grow on sulfide but grows on Fe2+, has apparently lost all genes involved in oxidation of sulfur compounds other than sqr. Instead, this organism possesses genes involved in assimilatory sulfate reduction, a trait that is unusual in GSB. The dissimilatory sulfite reductase (Dsr) enzyme system, which appears to be involved in elemental sulfur utilization, is found in all sulfide-utilizing strains except Chloroherpeton thalassium. The absence of Dsr enzymes in this early diverging GSB, in combination with phylogenetic analyses, suggests that the Dsr system in GSB could be a recent acquisition, which was obtained by lateral gene transfer in part from sulfideoxidizing bacteria and in part from sulfate-reducing bacteria. All thiosulfate-utilizing GSB strains have an identical sox gene cluster. The soxCD genes, which are found in certain other thiosulfate-utilizing organisms like Paracoccus pantotrophus, are absent from GSB. Flavocytochrome c, adenosine 5´-phosphosulfate reductase, ATP-sulfurylase, the Qmo complex, and other enzymes related to the utilization of sulfur compounds are found in some, but not all sulfide-utilizing strains. Even though different GSB strains superficially exhibit a similar sulfur oxidation phenotype, this may be caused by different combinations of enzymes. Thus, genome analyses have revealed that GSB have greater diversity in sulfur metabolism than previously suspected.

AB - Green sulfur bacteria (GSB) are anaerobic photoautotrophs that oxidize sulfide, elemental sulfur, thiosulfate, ferrous iron, and hydrogen for growth. We present here an analysis of the distribution and evolution of enzymes involved in oxidation of sulfur compounds in GSB based on genome sequence data from 12 strains. Sulfide:quinone reductase (SQR) is found in all strains. Chlorobium ferrooxidans, which cannot grow on sulfide but grows on Fe2+, has apparently lost all genes involved in oxidation of sulfur compounds other than sqr. Instead, this organism possesses genes involved in assimilatory sulfate reduction, a trait that is unusual in GSB. The dissimilatory sulfite reductase (Dsr) enzyme system, which appears to be involved in elemental sulfur utilization, is found in all sulfide-utilizing strains except Chloroherpeton thalassium. The absence of Dsr enzymes in this early diverging GSB, in combination with phylogenetic analyses, suggests that the Dsr system in GSB could be a recent acquisition, which was obtained by lateral gene transfer in part from sulfideoxidizing bacteria and in part from sulfate-reducing bacteria. All thiosulfate-utilizing GSB strains have an identical sox gene cluster. The soxCD genes, which are found in certain other thiosulfate-utilizing organisms like Paracoccus pantotrophus, are absent from GSB. Flavocytochrome c, adenosine 5´-phosphosulfate reductase, ATP-sulfurylase, the Qmo complex, and other enzymes related to the utilization of sulfur compounds are found in some, but not all sulfide-utilizing strains. Even though different GSB strains superficially exhibit a similar sulfur oxidation phenotype, this may be caused by different combinations of enzymes. Thus, genome analyses have revealed that GSB have greater diversity in sulfur metabolism than previously suspected.

U2 - 10.1007/978-3-540-72682-1_6

DO - 10.1007/978-3-540-72682-1_6

M3 - Book chapter

SN - 978-3-540-72679-1

SP - 60

EP - 76

BT - Microbial Sulfur Metabolism

PB - Springer

ER -