Genome annotation and comparative analyses of the odorant-binding proteins and chemosensory proteins in the pea aphid Acyrthosiphon pisum

TY - JOUR

T1 - Genome annotation and comparative analyses of the odorant-binding proteins and chemosensory proteins in the pea aphid Acyrthosiphon pisum

AU - Zhou, J. J.

AU - Vieira, F. G.

AU - He, X. L.

AU - Smadja, C.

AU - Liu, R.

AU - Rozas, J.

AU - Field, L. M.

PY - 2010/3

Y1 - 2010/3

N2 - Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are two families of small watersoluble proteins, abundant in the aqueous fluid surrounding olfactory receptor neurons in insect antennae. OBPs are involved in the first step of olfactory signal transduction, carrying airborne semiochemicals to the odorant receptors and can be classified into three groups: Classic OBPs, Plus-C OBPs and Atypical OBPs. Here, we identified and annotated genes encoding putative OBPs and CSPs in the pea aphid Acyrthosiphon pisum using bioinformatics. This identified genes encoding 13 Classic and two Plus-C OBPs and 13 CSPs. Homologous OBP sequences were also identified in nine other aphid species, allowing us to compare OBPs across several aphid and non-aphid species. We show that, although OBP sequences are divergent within a species and between different orders, there is a high similarity between orthologs within a range of aphid species. Furthermore, the phylogenetic relationships between OBP orthologs reflect the divergence of aphid evolution lineages. Our results support the 'birth-and-death' model as the major mechanism explaining aphid OBP sequence evolution, with the main force acting on the evolution being purifying selection.

AB - Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are two families of small watersoluble proteins, abundant in the aqueous fluid surrounding olfactory receptor neurons in insect antennae. OBPs are involved in the first step of olfactory signal transduction, carrying airborne semiochemicals to the odorant receptors and can be classified into three groups: Classic OBPs, Plus-C OBPs and Atypical OBPs. Here, we identified and annotated genes encoding putative OBPs and CSPs in the pea aphid Acyrthosiphon pisum using bioinformatics. This identified genes encoding 13 Classic and two Plus-C OBPs and 13 CSPs. Homologous OBP sequences were also identified in nine other aphid species, allowing us to compare OBPs across several aphid and non-aphid species. We show that, although OBP sequences are divergent within a species and between different orders, there is a high similarity between orthologs within a range of aphid species. Furthermore, the phylogenetic relationships between OBP orthologs reflect the divergence of aphid evolution lineages. Our results support the 'birth-and-death' model as the major mechanism explaining aphid OBP sequence evolution, with the main force acting on the evolution being purifying selection.

KW - Aphids

KW - Chemical ecology

KW - Gene evolution

KW - Olfaction

UR - http://www.scopus.com/inward/record.url?scp=77955592964&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2583.2009.00919.x

DO - 10.1111/j.1365-2583.2009.00919.x

M3 - Journal article

C2 - 20482644

AN - SCOPUS:77955592964

SN - 0962-1075

VL - 19

SP - 113

EP - 122

JO - Insect Molecular Biology

JF - Insect Molecular Biology

IS - SUPPL. 2

ER -