Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

C. Pedersen; S. K. Rasmussen; I. Linde-Laursen

doi:10.1139/g96-013

Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

C. Pedersen^*, S. K. Rasmussen, I. Linde-Laursen

^*Corresponding author af dette arbejde

116 Citationer (Scopus)

Abstract

The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.

Originalsprog	Engelsk
Tidsskrift	Genome
Vol/bind	39
Udgave nummer	1
Sider (fra-til)	93-104
Antal sider	12
ISSN	0831-2796
DOI	https://doi.org/10.1139/g96-013
Status	Udgivet - 1 jan. 1996

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@article{34795b2c2d09463aa21baa2d4cc86444,

title = "Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence",

abstract = "The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.",

keywords = "Chromosome identification, GAA-satellite sequence, Genome differentiation, Hordeum vulgare, Triticeae",

author = "C. Pedersen and Rasmussen, {S. K.} and I. Linde-Laursen",

year = "1996",

month = jan,

day = "1",

doi = "10.1139/g96-013",

language = "English",

volume = "39",

pages = "93--104",

journal = "Genome",

issn = "0831-2796",

publisher = "N R C Research Press",

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TY - JOUR

T1 - Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

AU - Pedersen, C.

AU - Rasmussen, S. K.

AU - Linde-Laursen, I.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.

AB - The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.

KW - Chromosome identification

KW - GAA-satellite sequence

KW - Genome differentiation

KW - Hordeum vulgare

KW - Triticeae

UR - http://www.scopus.com/inward/record.url?scp=0030087749&partnerID=8YFLogxK

U2 - 10.1139/g96-013

DO - 10.1139/g96-013

M3 - Journal article

C2 - 8851798

AN - SCOPUS:0030087749

SN - 0831-2796

VL - 39

SP - 93

EP - 104

JO - Genome

JF - Genome

IS - 1

ER -

Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence

Abstract

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