Genital form off pasteurellosis in breeding turkeys infected during artificial insemination and isolation off an unusual strain of Pasteurella multocida.

TY - JOUR

T1 - Genital form off pasteurellosis in breeding turkeys infected during artificial insemination and isolation off an unusual strain of Pasteurella multocida.

AU - Cariou, Nadine

AU - Christensen, Henrik

AU - Salandre, Olivier

AU - Albaric, Olivier

AU - Bisgaard, Magne

AU - Malher, Xavier

PY - 2013/9

Y1 - 2013/9

N2 - A genital and potentially fatal form of Pasteurella multocida infection was reported on two turkey-breeding farms on which birds were vaccinated against Pasteurella multocida. Both outbreaks were linked to the use of semen from young vaccinated toms with a history of respiratory pasteurellosis followed by treatment during rearing. Typing by agar gel immunodiffusion and rapid slide agglutination of P. multocida isolated from cloacal swabs was completed by multilocus sequence typing. Restriction enzyme analysis showed that that the isolates were clonal. They belonged to sequence type (ST) 30, described in chickens, cats, and ducks. This strain differed in sequence type from the ones used in the vaccine (ST8, ST60, ST53, and ST235), which might have limited its effectiveness. No contamination of the semen (ncontent> = 30) was found, suggesting fecal contamination during semen collection.

AB - A genital and potentially fatal form of Pasteurella multocida infection was reported on two turkey-breeding farms on which birds were vaccinated against Pasteurella multocida. Both outbreaks were linked to the use of semen from young vaccinated toms with a history of respiratory pasteurellosis followed by treatment during rearing. Typing by agar gel immunodiffusion and rapid slide agglutination of P. multocida isolated from cloacal swabs was completed by multilocus sequence typing. Restriction enzyme analysis showed that that the isolates were clonal. They belonged to sequence type (ST) 30, described in chickens, cats, and ducks. This strain differed in sequence type from the ones used in the vaccine (ST8, ST60, ST53, and ST235), which might have limited its effectiveness. No contamination of the semen (ncontent> = 30) was found, suggesting fecal contamination during semen collection.

KW - Faculty of Health and Medical Sciences

KW - pasteurellosis

KW - artificial insemination

KW - multilocus sequence typing

KW - turkey breeders

U2 - 10.1637/10471-121812-case.1

DO - 10.1637/10471-121812-case.1

M3 - Journal article

C2 - 24283141

SN - 0005-2086

VL - 57

SP - 693

EP - 697

JO - Avian Diseases

JF - Avian Diseases

IS - 3

ER -