Genetic selection and DNA sequences of 4.5S RNA homologs

TY - JOUR

T1 - Genetic selection and DNA sequences of 4.5S RNA homologs

AU - Brown, S

AU - Thon, G

AU - Tolentino, E

N1 - Keywords: Bacillus subtilis; Bacteria; Base Sequence; Cloning, Molecular; DNA, Ribosomal; Escherichia coli; Genes, Bacterial; Genetic Complementation Test; Genomic Library; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Ribosomal; Selection (Genetics); Sequence Homology, Nucleic Acid; Species Specificity

PY - 1989

Y1 - 1989

N2 - A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were determined. Since this approach does not require that the homologous genes hybridize with probes generated from the E. coli sequence, the sequences of the homologs were not all similar to the sequence of the E. coli gene. Despite the dissimilarity of the primary sequences of some of the homologs, all could be folded to obtain a similar structure.

AB - A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were determined. Since this approach does not require that the homologous genes hybridize with probes generated from the E. coli sequence, the sequences of the homologs were not all similar to the sequence of the E. coli gene. Despite the dissimilarity of the primary sequences of some of the homologs, all could be folded to obtain a similar structure.

M3 - Journal article

C2 - 2512280

SN - 0021-9193

VL - 171

SP - 6517

EP - 6520

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 12

ER -