Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland

Simon Andreasen; Tina Klitmøller Agander; Kristine Bjørndal; Daiva Erentaite; Steffen Heegaard; Stine R Larsen; Linea Cecilie Melchior; Qihua Tan; Benedicte Parm Ulhøi; Irene Wessel; Preben Homøe

doi:10.18632/oncotarget.24800

Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland

Simon Andreasen, Tina Klitmøller Agander, Kristine Bjørndal, Daiva Erentaite, Steffen Heegaard, Stine R Larsen, Linea Cecilie Melchior, Qihua Tan, Benedicte Parm Ulhøi, Irene Wessel, Preben Homøe

Institut for Klinisk Medicin

6 Citationer (Scopus)

46 Downloads (Pure)

Abstract

Adenoid cystic carcinoma (ACC) is among the most common salivary gland malignancies, and is notorious for its unpredictable clinical course with frequent local recurrences and metastatic spread. However, the molecular mechanisms for metastatic spread are poorly understood. This malignancy is known to frequently harbor gene fusions involving MYB, MYBL1, and NFIB, and to have a low mutational burden. Most studies have focused on primary tumors to understand the biology of ACC, but this has not revealed a genetic cause for metastatic dissemination in the majority of cases. Hence, other molecular mechanisms are likely to be involved. Here, we characterize the genetic and microRNA expressional landscape of primary ACC and corresponding metastatic lesions from 11 patients. FISH demonstrated preservation of MYB aberrations between primary tumors and metastases, and targeted next-generation sequencing identified mutations exclusive for the metastatic lesions in 3/11 cases (27.3%). Global microRNA profiling identified several differentially expressed miRNAs between primary ACC and metastases as compared to normal salivary gland tissue. Interestingly, individual tumor pairs differed in miRNA profile, but there was no general difference between primary ACCs and metastases. Collectively, we show that MYB and NFIB aberrations are consistently preserved in ACC metastatic lesions, and that additional mutations included in the 50-gene hotspot panel used are infrequently acquired by the metastatic lesions. In contrast, tumor pairs differ in microRNA expression and our data suggest that they are heterogeneous according to their microRNA profile. This adds an additional layer to the complex process of ACC metastatic spread.

Originalsprog	Engelsk
Tidsskrift	OncoTarget
Vol/bind	9
Udgave nummer	28
Sider (fra-til)	19675-19687
ISSN	1949-2553
DOI	https://doi.org/10.18632/oncotarget.24800
Status	Udgivet - 13 apr. 2018

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10.18632/oncotarget.24800Licens: CC BY

Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary glandForlagets udgivne version, 2,74 MBLicens: CC BY

Citationsformater

Andreasen, S., Agander, T. K., Bjørndal, K., Erentaite, D., Heegaard, S., Larsen, S. R., Melchior, L. C., Tan, Q., Ulhøi, B. P., Wessel, I., & Homøe, P. (2018). Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland. OncoTarget, 9(28), 19675-19687. https://doi.org/10.18632/oncotarget.24800

Andreasen, S, Agander, TK, Bjørndal, K, Erentaite, D, Heegaard, S, Larsen, SR, Melchior, LC, Tan, Q, Ulhøi, BP, Wessel, I & Homøe, P 2018, 'Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland', OncoTarget, bind 9, nr. 28, s. 19675-19687. https://doi.org/10.18632/oncotarget.24800

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title = "Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland",

abstract = "Adenoid cystic carcinoma (ACC) is among the most common salivary gland malignancies, and is notorious for its unpredictable clinical course with frequent local recurrences and metastatic spread. However, the molecular mechanisms for metastatic spread are poorly understood. This malignancy is known to frequently harbor gene fusions involving MYB, MYBL1, and NFIB, and to have a low mutational burden. Most studies have focused on primary tumors to understand the biology of ACC, but this has not revealed a genetic cause for metastatic dissemination in the majority of cases. Hence, other molecular mechanisms are likely to be involved. Here, we characterize the genetic and microRNA expressional landscape of primary ACC and corresponding metastatic lesions from 11 patients. FISH demonstrated preservation of MYB aberrations between primary tumors and metastases, and targeted next-generation sequencing identified mutations exclusive for the metastatic lesions in 3/11 cases (27.3%). Global microRNA profiling identified several differentially expressed miRNAs between primary ACC and metastases as compared to normal salivary gland tissue. Interestingly, individual tumor pairs differed in miRNA profile, but there was no general difference between primary ACCs and metastases. Collectively, we show that MYB and NFIB aberrations are consistently preserved in ACC metastatic lesions, and that additional mutations included in the 50-gene hotspot panel used are infrequently acquired by the metastatic lesions. In contrast, tumor pairs differ in microRNA expression and our data suggest that they are heterogeneous according to their microRNA profile. This adds an additional layer to the complex process of ACC metastatic spread.",

author = "Simon Andreasen and Agander, {Tina Klitm{\o}ller} and Kristine Bj{\o}rndal and Daiva Erentaite and Steffen Heegaard and Larsen, {Stine R} and Melchior, {Linea Cecilie} and Qihua Tan and Ulh{\o}i, {Benedicte Parm} and Irene Wessel and Preben Hom{\o}e",

year = "2018",

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doi = "10.18632/oncotarget.24800",

language = "English",

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TY - JOUR

T1 - Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland

AU - Andreasen, Simon

AU - Agander, Tina Klitmøller

AU - Bjørndal, Kristine

AU - Erentaite, Daiva

AU - Heegaard, Steffen

AU - Larsen, Stine R

AU - Melchior, Linea Cecilie

AU - Tan, Qihua

AU - Ulhøi, Benedicte Parm

AU - Wessel, Irene

AU - Homøe, Preben

PY - 2018/4/13

Y1 - 2018/4/13

N2 - Adenoid cystic carcinoma (ACC) is among the most common salivary gland malignancies, and is notorious for its unpredictable clinical course with frequent local recurrences and metastatic spread. However, the molecular mechanisms for metastatic spread are poorly understood. This malignancy is known to frequently harbor gene fusions involving MYB, MYBL1, and NFIB, and to have a low mutational burden. Most studies have focused on primary tumors to understand the biology of ACC, but this has not revealed a genetic cause for metastatic dissemination in the majority of cases. Hence, other molecular mechanisms are likely to be involved. Here, we characterize the genetic and microRNA expressional landscape of primary ACC and corresponding metastatic lesions from 11 patients. FISH demonstrated preservation of MYB aberrations between primary tumors and metastases, and targeted next-generation sequencing identified mutations exclusive for the metastatic lesions in 3/11 cases (27.3%). Global microRNA profiling identified several differentially expressed miRNAs between primary ACC and metastases as compared to normal salivary gland tissue. Interestingly, individual tumor pairs differed in miRNA profile, but there was no general difference between primary ACCs and metastases. Collectively, we show that MYB and NFIB aberrations are consistently preserved in ACC metastatic lesions, and that additional mutations included in the 50-gene hotspot panel used are infrequently acquired by the metastatic lesions. In contrast, tumor pairs differ in microRNA expression and our data suggest that they are heterogeneous according to their microRNA profile. This adds an additional layer to the complex process of ACC metastatic spread.

AB - Adenoid cystic carcinoma (ACC) is among the most common salivary gland malignancies, and is notorious for its unpredictable clinical course with frequent local recurrences and metastatic spread. However, the molecular mechanisms for metastatic spread are poorly understood. This malignancy is known to frequently harbor gene fusions involving MYB, MYBL1, and NFIB, and to have a low mutational burden. Most studies have focused on primary tumors to understand the biology of ACC, but this has not revealed a genetic cause for metastatic dissemination in the majority of cases. Hence, other molecular mechanisms are likely to be involved. Here, we characterize the genetic and microRNA expressional landscape of primary ACC and corresponding metastatic lesions from 11 patients. FISH demonstrated preservation of MYB aberrations between primary tumors and metastases, and targeted next-generation sequencing identified mutations exclusive for the metastatic lesions in 3/11 cases (27.3%). Global microRNA profiling identified several differentially expressed miRNAs between primary ACC and metastases as compared to normal salivary gland tissue. Interestingly, individual tumor pairs differed in miRNA profile, but there was no general difference between primary ACCs and metastases. Collectively, we show that MYB and NFIB aberrations are consistently preserved in ACC metastatic lesions, and that additional mutations included in the 50-gene hotspot panel used are infrequently acquired by the metastatic lesions. In contrast, tumor pairs differ in microRNA expression and our data suggest that they are heterogeneous according to their microRNA profile. This adds an additional layer to the complex process of ACC metastatic spread.

U2 - 10.18632/oncotarget.24800

DO - 10.18632/oncotarget.24800

M3 - Journal article

C2 - 29731974

SN - 1949-2553

VL - 9

SP - 19675

EP - 19687

JO - Oncotarget

JF - Oncotarget

IS - 28

ER -

Genetic rearrangements, hotspot mutations, and microRNA expression in the progression of metastatic adenoid cystic carcinoma of the salivary gland

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