Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

H Reynaldo López-Mirabal; Jakob R Winther; Morten C Kielland-Brandt

Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

H Reynaldo López-Mirabal, Jakob R Winther, Morten C Kielland-Brandt

Biomolecular Sciences

Abstract

The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant.
Udgivelsesdato: 2007-Dec

Originalsprog	Engelsk
Tidsskrift	Bioscience, Biotechnology, and Biochemistry
Vol/bind	71
Udgave nummer	12
Sider (fra-til)	2934-42
Antal sider	8
ISSN	0916-8451
Status	Udgivet - 2007

Citationsformater

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title = "Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.",

abstract = "The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec",

author = "L{\'o}pez-Mirabal, {H Reynaldo} and Winther, {Jakob R} and Kielland-Brandt, {Morten C}",

year = "2007",

language = "English",

volume = "71",

pages = "2934--42",

journal = "Bioscience, Biotechnology, and Biochemistry",

issn = "0916-8451",

publisher = "Taylor & Francis",

number = "12",

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TY - JOUR

T1 - Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

AU - López-Mirabal, H Reynaldo

AU - Winther, Jakob R

AU - Kielland-Brandt, Morten C

PY - 2007

Y1 - 2007

N2 - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec

AB - The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant. Udgivelsesdato: 2007-Dec

M3 - Journal article

C2 - 18071269

SN - 0916-8451

VL - 71

SP - 2934

EP - 2942

JO - Bioscience, Biotechnology, and Biochemistry

JF - Bioscience, Biotechnology, and Biochemistry

IS - 12

ER -