Generation of primary cultures of bovine brain endothelial cells and setup of cocultures with rat astrocytes

TY - JOUR

T1 - Generation of primary cultures of bovine brain endothelial cells and setup of cocultures with rat astrocytes

AU - Helms, Hans C

AU - Brodin, Birger

PY - 2014

Y1 - 2014

N2 - In vitro models of the blood-brain barrier are useful tools to study blood-brain barrier function as well as drug permeation from the systemic circulation to the brain parenchyma. However, a large number of the available in vitro models fail to reflect the tightness of the in vivo blood-brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes. The model displays a high electrical tightness and expresses blood-brain barrier marker proteins.

AB - In vitro models of the blood-brain barrier are useful tools to study blood-brain barrier function as well as drug permeation from the systemic circulation to the brain parenchyma. However, a large number of the available in vitro models fail to reflect the tightness of the in vivo blood-brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes. The model displays a high electrical tightness and expresses blood-brain barrier marker proteins.

U2 - 10.1007/978-1-4939-0320-7_30

DO - 10.1007/978-1-4939-0320-7_30

M3 - Journal article

C2 - 24510879

SN - 1940-6029

VL - 1135

SP - 365

EP - 382

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -