Generation of multipotent foregut stem cells from human pluripotent stem cells

TY - JOUR

T1 - Generation of multipotent foregut stem cells from human pluripotent stem cells

AU - Hannan, Nicholas R F

AU - Fordham, Robert P

AU - Syed, Yasir A

AU - Moignard, Victoria

AU - Berry, Andrew

AU - Bautista, Ruben

AU - Hanley, Neil A

AU - Jensen, Kim B

AU - Vallier, Ludovic

PY - 2013/10/15

Y1 - 2013/10/15

N2 - Summary Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.

AB - Summary Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value.

U2 - 10.1016/j.stemcr.2013.09.003

DO - 10.1016/j.stemcr.2013.09.003

M3 - Journal article

C2 - 24319665

SN - 1550-8943

VL - 1

SP - 293

EP - 306

JO - Stem Cell Reviews

JF - Stem Cell Reviews

IS - 4

ER -