Gene expression analysis of interferon-beta treatment in multiple sclerosis

F. Sellebjerg; P. Datta; J. Larsen; K. Rieneck; I. Alsing; A. Oturai; A. Svejgaard; Sorensen P. Soelberg; L.P. Ryder

Gene expression analysis of interferon-beta treatment in multiple sclerosis

F. Sellebjerg, P. Datta, J. Larsen, K. Rieneck, I. Alsing, A. Oturai, A. Svejgaard, Sorensen P. Soelberg, L.P. Ryder

34 Citationer (Scopus)

Abstract

Treatment with interferon-beta (IFN-beta) induces the expression of hundreds of genes in blood mononuclear cells, and the expression of several genes has been proposed as a marker of the effect of treatment with IFN-beta. However, to date no molecules have been identified that are stably induced by treatment with IFN-beta. We use DNA microarrays to study gene expression in 10 multiple sclerosis (MS) patients who began de novo treatment with IFN-beta. After the first injection of IFN-beta, the expression of 74 out of 3428 genes changed at least two-fold and statistically significantly (after Bonferroni correction). In contrast, we observed no persisting effects of IFN-beta on gene expression. Among the most strongly induced genes was MXA, which has been used in previous biomarker studies in MS. In addition, the study identified the induction of LGALS9 and TCIR1G, involved in negative regulation of T helper type I immunity and T-cell activation, as novel effects of IFN-beta therapy in MS
Udgivelsesdato: 2008/6

Originalsprog	Engelsk
Tidsskrift	Multiple Sclerosis
Vol/bind	14
Udgave nummer	5
Sider (fra-til)	615-621
Antal sider	6
ISSN	1352-4585
Status	Udgivet - 2008

Citationsformater

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title = "Gene expression analysis of interferon-beta treatment in multiple sclerosis",

abstract = "Treatment with interferon-beta (IFN-beta) induces the expression of hundreds of genes in blood mononuclear cells, and the expression of several genes has been proposed as a marker of the effect of treatment with IFN-beta. However, to date no molecules have been identified that are stably induced by treatment with IFN-beta. We use DNA microarrays to study gene expression in 10 multiple sclerosis (MS) patients who began de novo treatment with IFN-beta. After the first injection of IFN-beta, the expression of 74 out of 3428 genes changed at least two-fold and statistically significantly (after Bonferroni correction). In contrast, we observed no persisting effects of IFN-beta on gene expression. Among the most strongly induced genes was MXA, which has been used in previous biomarker studies in MS. In addition, the study identified the induction of LGALS9 and TCIR1G, involved in negative regulation of T helper type I immunity and T-cell activation, as novel effects of IFN-beta therapy in MS Udgivelsesdato: 2008/6",

author = "F. Sellebjerg and P. Datta and J. Larsen and K. Rieneck and I. Alsing and A. Oturai and A. Svejgaard and Soelberg, {Sorensen P.} and L.P. Ryder",

year = "2008",

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journal = "Multiple Sclerosis Journal",

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TY - JOUR

T1 - Gene expression analysis of interferon-beta treatment in multiple sclerosis

AU - Sellebjerg, F.

AU - Datta, P.

AU - Larsen, J.

AU - Rieneck, K.

AU - Alsing, I.

AU - Oturai, A.

AU - Svejgaard, A.

AU - Soelberg, Sorensen P.

AU - Ryder, L.P.

PY - 2008

Y1 - 2008

N2 - Treatment with interferon-beta (IFN-beta) induces the expression of hundreds of genes in blood mononuclear cells, and the expression of several genes has been proposed as a marker of the effect of treatment with IFN-beta. However, to date no molecules have been identified that are stably induced by treatment with IFN-beta. We use DNA microarrays to study gene expression in 10 multiple sclerosis (MS) patients who began de novo treatment with IFN-beta. After the first injection of IFN-beta, the expression of 74 out of 3428 genes changed at least two-fold and statistically significantly (after Bonferroni correction). In contrast, we observed no persisting effects of IFN-beta on gene expression. Among the most strongly induced genes was MXA, which has been used in previous biomarker studies in MS. In addition, the study identified the induction of LGALS9 and TCIR1G, involved in negative regulation of T helper type I immunity and T-cell activation, as novel effects of IFN-beta therapy in MS Udgivelsesdato: 2008/6

AB - Treatment with interferon-beta (IFN-beta) induces the expression of hundreds of genes in blood mononuclear cells, and the expression of several genes has been proposed as a marker of the effect of treatment with IFN-beta. However, to date no molecules have been identified that are stably induced by treatment with IFN-beta. We use DNA microarrays to study gene expression in 10 multiple sclerosis (MS) patients who began de novo treatment with IFN-beta. After the first injection of IFN-beta, the expression of 74 out of 3428 genes changed at least two-fold and statistically significantly (after Bonferroni correction). In contrast, we observed no persisting effects of IFN-beta on gene expression. Among the most strongly induced genes was MXA, which has been used in previous biomarker studies in MS. In addition, the study identified the induction of LGALS9 and TCIR1G, involved in negative regulation of T helper type I immunity and T-cell activation, as novel effects of IFN-beta therapy in MS Udgivelsesdato: 2008/6

M3 - Journal article

SN - 1352-4585

VL - 14

SP - 615

EP - 621

JO - Multiple Sclerosis Journal

JF - Multiple Sclerosis Journal

IS - 5

ER -