Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology

Eduardo Antonio Della Pia; Randi Westh Hansen; Manuela Zoonens; Karen L. Martinez

doi:10.1007/s00232-014-9663-y

Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology

Eduardo Antonio Della Pia, Randi Westh Hansen, Manuela Zoonens, Karen L. Martinez^*

^*Corresponding author af dette arbejde

Kemisk Institut

13 Citationer (Scopus)

Abstract

Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.

Originalsprog	Engelsk
Tidsskrift	Journal of Membrane Biology
Vol/bind	247
Udgave nummer	9-10
Sider (fra-til)	815-826
Antal sider	12
ISSN	0022-2631
DOI	https://doi.org/10.1007/s00232-014-9663-y
Status	Udgivet - 2014

Adgang til dokumentet

10.1007/s00232-014-9663-y

Citationsformater

@article{2e2ef0e7d4b74aaeb2e49e9e6bc8e45e,

title = "Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology",

abstract = "Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.",

keywords = "Biosensors, Multiple functionalization, Protein immobilization, Protein labeling, Self-assembly",

author = "{Della Pia}, {Eduardo Antonio} and Hansen, {Randi Westh} and Manuela Zoonens and Martinez, {Karen L.}",

year = "2014",

doi = "10.1007/s00232-014-9663-y",

language = "English",

volume = "247",

pages = "815--826",

journal = "Journal of Membrane Biology",

issn = "0022-2631",

publisher = "Springer",

number = "9-10",

}

TY - JOUR

T1 - Functionalized amphipols

T2 - a versatile toolbox suitable for applications of membrane proteins in synthetic biology

AU - Della Pia, Eduardo Antonio

AU - Hansen, Randi Westh

AU - Zoonens, Manuela

AU - Martinez, Karen L.

PY - 2014

Y1 - 2014

N2 - Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.

AB - Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.

KW - Biosensors

KW - Multiple functionalization

KW - Protein immobilization

KW - Protein labeling

KW - Self-assembly

U2 - 10.1007/s00232-014-9663-y

DO - 10.1007/s00232-014-9663-y

M3 - Journal article

C2 - 24728227

AN - SCOPUS:84910113035

SN - 0022-2631

VL - 247

SP - 815

EP - 826

JO - Journal of Membrane Biology

JF - Journal of Membrane Biology

IS - 9-10

ER -

Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater