TY - JOUR
T1 - Functional improvement of antibody fragments using a novel phage coat protein III fusion system
AU - Jensen, Kim Bak
AU - Larsen, Martin
AU - Pedersen, Jesper Søndergaard
AU - Christensen, Peter Astrup
AU - Alvarez-Vallina, Luis
AU - Goletz, Steffen
AU - Clark, Brian F C
AU - Kristensen, Peter
PY - 2002/11/8
Y1 - 2002/11/8
N2 - Functional expressions of proteins often depend on the presence of host specific factors. Frequently recombinant expression strategies of proteins in foreign hosts, such as bacteria, have been associated with poor yields or significant loss of functionality. Improvements in the performance of heterologous expression systems will benefit present-day quests in structural and functional genomics where high amounts of active protein are required. One example, which has been the subject of considerable interest, is recombinant antibodies or fragments thereof as expressions of these in bacteria constitute an easy and inexpensive method compared to hybridoma cultures. Such approaches have, however, often suffered from low yields and poor functionality. A general method is described here which enables expressions of functional antibody fragments when fused to the amino-terminal domain(s) of the filamentous phage coat protein III. Furthermore, it will be shown that the observed effect is neither due to improved stability nor increased avidity.
AB - Functional expressions of proteins often depend on the presence of host specific factors. Frequently recombinant expression strategies of proteins in foreign hosts, such as bacteria, have been associated with poor yields or significant loss of functionality. Improvements in the performance of heterologous expression systems will benefit present-day quests in structural and functional genomics where high amounts of active protein are required. One example, which has been the subject of considerable interest, is recombinant antibodies or fragments thereof as expressions of these in bacteria constitute an easy and inexpensive method compared to hybridoma cultures. Such approaches have, however, often suffered from low yields and poor functionality. A general method is described here which enables expressions of functional antibody fragments when fused to the amino-terminal domain(s) of the filamentous phage coat protein III. Furthermore, it will be shown that the observed effect is neither due to improved stability nor increased avidity.
KW - Base Sequence
KW - Capsid Proteins/chemistry
KW - Chromatography, Gel
KW - DNA Primers
KW - Enzyme-Linked Immunosorbent Assay
KW - Immunoglobulin Fragments/immunology
KW - Inovirus/immunology
KW - Recombinant Fusion Proteins/chemistry
M3 - Journal article
C2 - 12408989
SN - 1522-4724
VL - 298
SP - 566
EP - 573
JO - Molecular Cell Biology Research Communications
JF - Molecular Cell Biology Research Communications
IS - 4
ER -