@article{15c92bd0b0a911ddb538000ea68e967b,
title = "Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells",
abstract = "In the present experiments we have explored the possibilities of a modified immunoadsorbent technique to select for (1) mutagenized T cell receptor (Tcr) negative variants of Jurkat T lymphoma cells and (2) purified CD-4+ or CD-8+ T lymphocytes. The basic principle was to make large numbers of immunoglobulin (Ig) negative T cells Ig+ by T cell subset-specific monoclonal antibodies (mAb), and to select such cells on Ig anti-Ig columns. Our results demonstrated that Thy-1+, Fc receptor positive, antigen-specific T cells regulate the immune response in mice nonresponders to pork insulin, and the {"}autologous{"} mixed lymphocyte reaction. In addition, the immunoadsorbent method very efficiently selects Tcr/CD-3- variants from mutagenized Jurkat cell populations incubated with anti-CD3 mAb. The described method is easy and quick and can fractionate large numbers of cells; it is the {"}poor-man's cell sorter.{"} The most important finding is the demonstration of antigen-specific Thy-1+, CD-8+, and Fc receptor+ T suppressor cell that apparently react with antigen in a non-major histocompatibility complex-restricted manner.",
author = "B Rubin and C Geisler and J Kuhlmann and T Plesner",
note = "Keywords: Animals; Antigens; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Cell Fractionation; Immunosorbent Techniques; Insulin; Lymphocyte Culture Test, Mixed; Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; T-Lymphocytes; T-Lymphocytes, Helper-Inducer; T-Lymphocytes, Regulatory",
year = "1989",
language = "English",
volume = "119",
pages = "327--40",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press",
number = "2",
}