Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

J. W. Helge; D. Bentley; P. Schjerling; M. Willer; M. J. Gibala; J. Franch; M. A. Tapia-Laliena; J. R. Daugaard; J. L. Andersen

doi:10.1007/s00421-007-0479-7

Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

J. W. Helge^*, D. Bentley, P. Schjerling, M. Willer, M. J. Gibala, J. Franch, M. A. Tapia-Laliena, J. R. Daugaard, J. L. Andersen

^*Corresponding author af dette arbejde

8 Citationer (Scopus)

Abstract

Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPARα expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5 h in total), while the other leg (UT) served as control. During the 4 weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30 min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P < 0.05) by 15% in the trained, but not the control leg in both groups. During exercise respiratory exchange ratio was lower in FAT (0.86 ± 0.01, 0.83 ± 0.01, mean ± SEM) than CHO (0.96 ± 0.02, 0.94 ± 0.03) and in UT than T legs, respectively. The PPARα protein (144 ± 44, 104 ± 28, 79 ± 15, 79 ± 14, % of pre level) and PPARα mRNA (69 ± [2, 2], 78 ± [7, 6], 92 ± [22, 18], 106 ± [21, 18], % of pre level, geometric mean ± SEM) expression remained unchanged by diet and training in FAT (UT, T) and CHO (UT, T), respectively. After the training and diet CS, HAD, PPARα, UCP2, UCP3 and mFABP mRNA content remained unchanged, whereas GLUT4 mRNA was lower in both groups and LDHA mRNA was lower (P < 0.05) only in FAT. In conclusion: 4 weeks one leg knee extensor training did not affect PPARα protein or mRNA expression. Furthermore, higher fat oxidation during exercise after fat rich diet was not accompanied by an increased PPARα protein or mRNA expression after 4 weeks.

Originalsprog	Engelsk
Tidsskrift	European Journal of Applied Physiology
Vol/bind	101
Udgave nummer	1
Sider (fra-til)	105-114
Antal sider	10
ISSN	1439-6319
DOI	https://doi.org/10.1007/s00421-007-0479-7
Status	Udgivet - 1 sep. 2007

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title = "Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle",

abstract = "Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPARα expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5 h in total), while the other leg (UT) served as control. During the 4 weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30 min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P < 0.05) by 15% in the trained, but not the control leg in both groups. During exercise respiratory exchange ratio was lower in FAT (0.86 ± 0.01, 0.83 ± 0.01, mean ± SEM) than CHO (0.96 ± 0.02, 0.94 ± 0.03) and in UT than T legs, respectively. The PPARα protein (144 ± 44, 104 ± 28, 79 ± 15, 79 ± 14, % of pre level) and PPARα mRNA (69 ± [2, 2], 78 ± [7, 6], 92 ± [22, 18], 106 ± [21, 18], % of pre level, geometric mean ± SEM) expression remained unchanged by diet and training in FAT (UT, T) and CHO (UT, T), respectively. After the training and diet CS, HAD, PPARα, UCP2, UCP3 and mFABP mRNA content remained unchanged, whereas GLUT4 mRNA was lower in both groups and LDHA mRNA was lower (P < 0.05) only in FAT. In conclusion: 4 weeks one leg knee extensor training did not affect PPARα protein or mRNA expression. Furthermore, higher fat oxidation during exercise after fat rich diet was not accompanied by an increased PPARα protein or mRNA expression after 4 weeks.",

keywords = "Enzyme activity, Exercise, Fibre types, Lipids, One-leg model",

author = "Helge, {J. W.} and D. Bentley and P. Schjerling and M. Willer and Gibala, {M. J.} and J. Franch and Tapia-Laliena, {M. A.} and Daugaard, {J. R.} and Andersen, {J. L.}",

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doi = "10.1007/s00421-007-0479-7",

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T1 - Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

AU - Helge, J. W.

AU - Bentley, D.

AU - Schjerling, P.

AU - Willer, M.

AU - Gibala, M. J.

AU - Franch, J.

AU - Tapia-Laliena, M. A.

AU - Daugaard, J. R.

AU - Andersen, J. L.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPARα expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5 h in total), while the other leg (UT) served as control. During the 4 weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30 min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P < 0.05) by 15% in the trained, but not the control leg in both groups. During exercise respiratory exchange ratio was lower in FAT (0.86 ± 0.01, 0.83 ± 0.01, mean ± SEM) than CHO (0.96 ± 0.02, 0.94 ± 0.03) and in UT than T legs, respectively. The PPARα protein (144 ± 44, 104 ± 28, 79 ± 15, 79 ± 14, % of pre level) and PPARα mRNA (69 ± [2, 2], 78 ± [7, 6], 92 ± [22, 18], 106 ± [21, 18], % of pre level, geometric mean ± SEM) expression remained unchanged by diet and training in FAT (UT, T) and CHO (UT, T), respectively. After the training and diet CS, HAD, PPARα, UCP2, UCP3 and mFABP mRNA content remained unchanged, whereas GLUT4 mRNA was lower in both groups and LDHA mRNA was lower (P < 0.05) only in FAT. In conclusion: 4 weeks one leg knee extensor training did not affect PPARα protein or mRNA expression. Furthermore, higher fat oxidation during exercise after fat rich diet was not accompanied by an increased PPARα protein or mRNA expression after 4 weeks.

AB - Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPARα expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5 h in total), while the other leg (UT) served as control. During the 4 weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30 min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P < 0.05) by 15% in the trained, but not the control leg in both groups. During exercise respiratory exchange ratio was lower in FAT (0.86 ± 0.01, 0.83 ± 0.01, mean ± SEM) than CHO (0.96 ± 0.02, 0.94 ± 0.03) and in UT than T legs, respectively. The PPARα protein (144 ± 44, 104 ± 28, 79 ± 15, 79 ± 14, % of pre level) and PPARα mRNA (69 ± [2, 2], 78 ± [7, 6], 92 ± [22, 18], 106 ± [21, 18], % of pre level, geometric mean ± SEM) expression remained unchanged by diet and training in FAT (UT, T) and CHO (UT, T), respectively. After the training and diet CS, HAD, PPARα, UCP2, UCP3 and mFABP mRNA content remained unchanged, whereas GLUT4 mRNA was lower in both groups and LDHA mRNA was lower (P < 0.05) only in FAT. In conclusion: 4 weeks one leg knee extensor training did not affect PPARα protein or mRNA expression. Furthermore, higher fat oxidation during exercise after fat rich diet was not accompanied by an increased PPARα protein or mRNA expression after 4 weeks.

KW - Enzyme activity

KW - Exercise

KW - Fibre types

KW - Lipids

KW - One-leg model

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U2 - 10.1007/s00421-007-0479-7

DO - 10.1007/s00421-007-0479-7

M3 - Journal article

C2 - 17530276

AN - SCOPUS:34548286306

SN - 1439-6319

VL - 101

SP - 105

EP - 114

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

IS - 1

ER -

Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

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