TY - JOUR
T1 - Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici
AU - Schuster, M
AU - Kilaru, S
AU - Latz, Meike Anna Christine
AU - Steinberg, G
N1 - Copyright © 2015. Published by Elsevier Inc.
PY - 2015
Y1 - 2015
N2 - The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.
AB - The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.
KW - Ascomycota
KW - Cytoskeletal Proteins
KW - Genes, Reporter
KW - Luminescent Proteins
KW - Microtubules
KW - Optical Imaging
KW - Recombinant Fusion Proteins
KW - Staining and Labeling
U2 - 10.1016/j.fgb.2015.03.005
DO - 10.1016/j.fgb.2015.03.005
M3 - Journal article
C2 - 25857261
SN - 1087-1845
VL - 79
SP - 141
EP - 149
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
ER -