Fluorescent markers of the endocytic pathway in Zymoseptoria tritici

TY - JOUR

T1 - Fluorescent markers of the endocytic pathway in Zymoseptoria tritici

AU - Kilaru, S

AU - Schuster, M

AU - Latz, M

AU - Guo, M

AU - Steinberg, G

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015

Y1 - 2015

N2 - Hyphal growth in filamentous fungi is supported by the uptake (endocytosis) and recycling of membranes and associated proteins at the growing tip. An increasing body of published evidence in various fungi demonstrates that this process is of essential importance for fungal growth and pathogenicity. Here, we introduce fluorescent markers to visualize the endocytic pathway in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein (eGFP) to the actin-binding protein fimbrin (ZtFim1), which is located in actin patches that are formed at the plasma membrane and are participating in endocytic uptake at the cell surface. In addition, we tagged early endosomes by eGFP-labelling a Rab5-homologue (ZtRab5) and late endosomes and vacuoles by expressing eGFP-Rab7 homologue (ZtRab7). Using fluorescent dyes and live cell imaging we confirmed the dynamic behavior and localization of these markers. This set of molecular tools enables an in-depth phenotypic analysis of Z. tritici mutant strains thereby supporting new strategies towards the goal of controlling wheat against Z. tritici.

AB - Hyphal growth in filamentous fungi is supported by the uptake (endocytosis) and recycling of membranes and associated proteins at the growing tip. An increasing body of published evidence in various fungi demonstrates that this process is of essential importance for fungal growth and pathogenicity. Here, we introduce fluorescent markers to visualize the endocytic pathway in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein (eGFP) to the actin-binding protein fimbrin (ZtFim1), which is located in actin patches that are formed at the plasma membrane and are participating in endocytic uptake at the cell surface. In addition, we tagged early endosomes by eGFP-labelling a Rab5-homologue (ZtRab5) and late endosomes and vacuoles by expressing eGFP-Rab7 homologue (ZtRab7). Using fluorescent dyes and live cell imaging we confirmed the dynamic behavior and localization of these markers. This set of molecular tools enables an in-depth phenotypic analysis of Z. tritici mutant strains thereby supporting new strategies towards the goal of controlling wheat against Z. tritici.

KW - Ascomycota

KW - Endocytosis

KW - Genes, Reporter

KW - Green Fluorescent Proteins

KW - Membrane Glycoproteins

KW - Microfilament Proteins

KW - Optical Imaging

KW - Recombinant Fusion Proteins

KW - Staining and Labeling

U2 - 10.1016/j.fgb.2015.03.019

DO - 10.1016/j.fgb.2015.03.019

M3 - Journal article

C2 - 26092801

SN - 1087-1845

VL - 79

SP - 150

EP - 157

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -