Fluorescence quenching and ligand binding: A critical discussion of a popular methodology

TY - JOUR

T1 - Fluorescence quenching and ligand binding: A critical discussion of a popular methodology

AU - van de Weert, Marco

AU - Stella, Lorenzo

PY - 2011/7/13

Y1 - 2011/7/13

N2 - In recent years fluorescence quenching has become a popular tool to investigate various aspects of ligand binding. Unfortunately, various pitfalls are often overlooked in a large number of papers, published in many different journals. In this criticism we discuss a number of possible mistakes and show how they may affect the data and their analysis. Moreover, we point to problems in the understanding of the fundamentals of fluorescence quenching, and show direct contradictions within many of these papers. This review hopefully contributes to a re-appraisal of the published literature and to a more appropriate use of fluorescence quenching to study ligand binding.

AB - In recent years fluorescence quenching has become a popular tool to investigate various aspects of ligand binding. Unfortunately, various pitfalls are often overlooked in a large number of papers, published in many different journals. In this criticism we discuss a number of possible mistakes and show how they may affect the data and their analysis. Moreover, we point to problems in the understanding of the fundamentals of fluorescence quenching, and show direct contradictions within many of these papers. This review hopefully contributes to a re-appraisal of the published literature and to a more appropriate use of fluorescence quenching to study ligand binding.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.molstruc.2011.05.023

DO - 10.1016/j.molstruc.2011.05.023

M3 - Journal article

SN - 0166-1280

VL - 998

SP - 144

EP - 150

JO - Journal of Molecular Structure: THEOCHEM

JF - Journal of Molecular Structure: THEOCHEM

IS - 1-3

ER -