Fluorescence in situ hybridization for detection of small RNAs on frozen tissue sections

5 Citationer (Scopus)

Abstract

MicroRNAs are an abundant class of small noncoding RNAs that regulate and fine-tune the expression of protein-coding genes. Each microRNA regulates around 100 genes, and they are mostly conserved and abundant within the multicellular organisms. Even though microRNAs have a role in many biological processes and diseases, the function of each single microRNA is still yet to be explored in all tissues and cells they are present. Therefore, an efficient in situ hybridization method, combining locked nucleic acid technology and tyramide signal amplification system, has been developed and presented for detection of microRNAs in frozen section at a cellular resolution and with high sensitivity.

OriginalsprogEngelsk
TitelMethods in Molecular Biology- Methods and Protocols : In Situ Hybridization Protocols
RedaktørerBoye Schnack Nielsen
Antal sider8
Vol/bind1211
ForlagSpringer
Publikationsdato2014
Sider95-102
ISBN (Trykt)978-1-4939-1458-6
ISBN (Elektronisk)978-1-4939-1459-3
DOI
StatusUdgivet - 2014
NavnMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

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