FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation

Pranjul Jaykumar Shah; Indumathi Vedarethinam; Dorota Kwasny; Lars Andresen; Søren Skov; Asli Silahtaroglu; Zeynep Tümer; Maria Dimaki; Winnie Edith Svendsen

doi:10.3390/mi2020116

FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation

Pranjul Jaykumar Shah, Indumathi Vedarethinam, Dorota Kwasny, Lars Andresen, Søren Skov, Asli Silahtaroglu, Zeynep Tümer, Maria Dimaki, Winnie Edith Svendsen

Institut for Cellulær og Molekylær Medicin

10 Citationer (Scopus)

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Abstract

We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3-4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.

Originalsprog	Engelsk
Tidsskrift	Micromachines
Vol/bind	2
Udgave nummer	2
Sider (fra-til)	116-128
Antal sider	13
ISSN	2072-666X
DOI	https://doi.org/10.3390/mi2020116
Status	Udgivet - 2011

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10.3390/mi2020116

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Citationsformater

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abstract = "We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3-4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.",

author = "Shah, {Pranjul Jaykumar} and Indumathi Vedarethinam and Dorota Kwasny and Lars Andresen and S{\o}ren Skov and Asli Silahtaroglu and Zeynep T{\"u}mer and Maria Dimaki and Svendsen, {Winnie Edith}",

year = "2011",

doi = "10.3390/mi2020116",

language = "English",

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T1 - FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation

AU - Shah, Pranjul Jaykumar

AU - Vedarethinam, Indumathi

AU - Kwasny, Dorota

AU - Andresen, Lars

AU - Skov, Søren

AU - Silahtaroglu, Asli

AU - Tümer, Zeynep

AU - Dimaki, Maria

AU - Svendsen, Winnie Edith

PY - 2011

Y1 - 2011

N2 - We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3-4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.

AB - We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics). Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3-4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis. The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.

U2 - 10.3390/mi2020116

DO - 10.3390/mi2020116

M3 - Journal article

SN - 2072-666X

VL - 2

SP - 116

EP - 128

JO - Micromachines

JF - Micromachines

IS - 2

ER -

FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation

Abstract

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