Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

Tanja L. Jensen; Maria K. Kiersgaard; Lars F. Mikkelsen; Dorte B. Sørensen

doi:10.1177/0023677218824373

Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

Tanja L. Jensen, Maria K. Kiersgaard, Lars F. Mikkelsen, Dorte B. Sørensen^*

^*Corresponding author af dette arbejde

4 Citationer (Scopus)

Abstract

Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.

Originalsprog	Engelsk
Tidsskrift	Laboratory Animals
ISSN	0023-6772
DOI	https://doi.org/10.1177/0023677218824373
Status	Udgivet - 1 dec. 2019

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10.1177/0023677218824373

Citationsformater

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title = "Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare",

abstract = "Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.",

keywords = "Fasting, mouse, refinement, welfare",

author = "Jensen, {Tanja L.} and Kiersgaard, {Maria K.} and Mikkelsen, {Lars F.} and S{\o}rensen, {Dorte B.}",

year = "2019",

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journal = "Laboratory Animals",

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T1 - Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

AU - Jensen, Tanja L.

AU - Kiersgaard, Maria K.

AU - Mikkelsen, Lars F.

AU - Sørensen, Dorte B.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.

AB - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.

KW - Fasting

KW - mouse

KW - refinement

KW - welfare

U2 - 10.1177/0023677218824373

DO - 10.1177/0023677218824373

M3 - Journal article

C2 - 30741083

AN - SCOPUS:85061611693

SN - 0023-6772

JO - Laboratory Animals

JF - Laboratory Animals

ER -

Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare

Abstract

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