TY - JOUR
T1 - Extraction of polycyclic aromatic hydrocarbons from polyhydroxyalkanoates before gas chromatography/mass spectrometry analysis
AU - Cavaliere, Chiara
AU - Montone, Carmela Maria
AU - Capriotti, Anna Laura
AU - La Barbera, Giorgia
AU - Piovesana, Susy
AU - Rotatori, Mauro
AU - Valentino, Francesco
AU - Laganà, Aldo
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Among the organic contaminants that could pass from waste to polyhydroxyalkanoates (PHAs), there are the polycyclic aromatic hydrocarbons (PAHs). For this reason, we have developed a rapid analytical method for the determination of sixteen PAHs in PHAs. PAHs were extracted by n-hexane, after matrix dispersion and crumbling into sand; the extract was purified by solid phase extraction using florisil as adsorbent. Recoveries in the range of 89–101% were obtained for the deuterated analytes, except for the two with the lowest molecular weight. Trueness between 92% and 108% and within-laboratory precision (expressed as relative standard deviation) ≤ 18% were estimated for all the analytes. Gas chromatography/mass spectrometry was used for analyte determination. Method limits of quantification were suitable to assure that PAH presence in PHA biolpolymers is much below the limits set by European law for plastic materials. Indeed, analysis of two different PHA samples showed that contamination is limited to few compounds at non-concerning levels.
AB - Among the organic contaminants that could pass from waste to polyhydroxyalkanoates (PHAs), there are the polycyclic aromatic hydrocarbons (PAHs). For this reason, we have developed a rapid analytical method for the determination of sixteen PAHs in PHAs. PAHs were extracted by n-hexane, after matrix dispersion and crumbling into sand; the extract was purified by solid phase extraction using florisil as adsorbent. Recoveries in the range of 89–101% were obtained for the deuterated analytes, except for the two with the lowest molecular weight. Trueness between 92% and 108% and within-laboratory precision (expressed as relative standard deviation) ≤ 18% were estimated for all the analytes. Gas chromatography/mass spectrometry was used for analyte determination. Method limits of quantification were suitable to assure that PAH presence in PHA biolpolymers is much below the limits set by European law for plastic materials. Indeed, analysis of two different PHA samples showed that contamination is limited to few compounds at non-concerning levels.
KW - Biopolymers
KW - Florisil clean-up
KW - Gas chromatography/mass spectrometry
KW - Isotopic dilution
KW - Polycyclic aromatic hydrocarbons
KW - Polyhydroxyalkanoates
UR - http://www.scopus.com/inward/record.url?scp=85048814267&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2018.06.038
DO - 10.1016/j.talanta.2018.06.038
M3 - Journal article
C2 - 30029430
AN - SCOPUS:85048814267
SN - 0039-9140
VL - 188
SP - 671
EP - 675
JO - Talanta
JF - Talanta
ER -