TY - JOUR
T1 - Extracellular calcium sensing in rat aortic vascular smooth muscle cells
AU - Smajilovic, Sanela
AU - Hansen, Jakob Lerche
AU - Christoffersen, Tue E.H.
AU - Lewin, Ewa
AU - Sheikh, Søren P.
AU - Terwilliger, E. F.
AU - Brown, Edward M.
AU - Haunso, Stig
AU - Tfelt-Hansen, Jacob
PY - 2006/10/6
Y1 - 2006/10/6
N2 - Extracellular calcium (Ca2 +
o) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca2 +
o stimulates proliferation of the cells. The effects of Ca2 +
o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca2 +
o-induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca2 +
o-induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium.
AB - Extracellular calcium (Ca2 +
o) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca2 +
o stimulates proliferation of the cells. The effects of Ca2 +
o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca2 +
o-induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca2 +
o-induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium.
KW - Atherosclerosis
KW - Calcium-sensing receptor
KW - ERK1/2
KW - Proliferation
KW - Vascular smooth muscle cell
UR - http://www.scopus.com/inward/record.url?scp=33748147021&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.07.192
DO - 10.1016/j.bbrc.2006.07.192
M3 - Journal article
C2 - 16919596
AN - SCOPUS:33748147021
SN - 0006-291X
VL - 348
SP - 1215
EP - 1223
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -