Expression of SMAD signal transduction molecules in the pancreas

Michael Brorson; D. Hougaard; Jens Høiriis Nielsen; D. Tornehave; Lars-Inge Larsson

doi:10.1007/s004180100316

Expression of SMAD signal transduction molecules in the pancreas

Michael Brorson, D. Hougaard, Jens Høiriis Nielsen, D. Tornehave, Lars-Inge Larsson

Inflammation, Metabolism and Oxidation

23 Citationer (Scopus)

Abstract

Members of the TGF-beta superfamily of cytokines have been implicated in pancreatic cancer, pancreatitis and in regulation and differentiation of pancreatic endocrine and exocrine cells. Different TGF-beta members signal through phosphorylation of different signal transduction proteins, which eventually form oligomers with SMAD 4 and translocate to the nucleus. Reverse transcriptase-polymerase chain reaction showed that SMADs 1, 2 and 4 are expressed in pancreatic islets. Immunostaining revealed that SMAD 1 and 4 predominantly were expressed by islet insulin and glucagon cells. Since SMAD 1 is known to transduce signals from receptors binding bone morphogenetic protein (BMP) these results indicate a previously unknown role of BMP-like ligands in islet function.

Originalsprog	Engelsk
Tidsskrift	Histochemistry and Cell Biology
Vol/bind	116
Udgave nummer	3
Sider (fra-til)	263-7
Antal sider	5
ISSN	0948-6143
DOI	https://doi.org/10.1007/s004180100316
Status	Udgivet - 2001

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10.1007/s004180100316

Citationsformater

@article{c98728e33ff342869eff856eba6d616e,

title = "Expression of SMAD signal transduction molecules in the pancreas",

abstract = "Members of the TGF-beta superfamily of cytokines have been implicated in pancreatic cancer, pancreatitis and in regulation and differentiation of pancreatic endocrine and exocrine cells. Different TGF-beta members signal through phosphorylation of different signal transduction proteins, which eventually form oligomers with SMAD 4 and translocate to the nucleus. Reverse transcriptase-polymerase chain reaction showed that SMADs 1, 2 and 4 are expressed in pancreatic islets. Immunostaining revealed that SMAD 1 and 4 predominantly were expressed by islet insulin and glucagon cells. Since SMAD 1 is known to transduce signals from receptors binding bone morphogenetic protein (BMP) these results indicate a previously unknown role of BMP-like ligands in islet function.",

keywords = "Animals, Animals, Newborn, DNA-Binding Proteins, Fluorescein-5-isothiocyanate, Fluorescent Antibody Technique, Gene Expression, Immunohistochemistry, Pancreas, RNA, Messenger, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Smad Proteins, Smad1 Protein, Smad2 Protein, Smad4 Protein, Trans-Activators",

author = "Michael Brorson and D. Hougaard and Nielsen, {Jens H{\o}iriis} and D. Tornehave and Lars-Inge Larsson",

year = "2001",

doi = "10.1007/s004180100316",

language = "English",

volume = "116",

pages = "263--7",

journal = "Histochemistry and Cell Biology",

issn = "0948-6143",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Expression of SMAD signal transduction molecules in the pancreas

AU - Brorson, Michael

AU - Hougaard, D.

AU - Nielsen, Jens Høiriis

AU - Tornehave, D.

AU - Larsson, Lars-Inge

PY - 2001

Y1 - 2001

N2 - Members of the TGF-beta superfamily of cytokines have been implicated in pancreatic cancer, pancreatitis and in regulation and differentiation of pancreatic endocrine and exocrine cells. Different TGF-beta members signal through phosphorylation of different signal transduction proteins, which eventually form oligomers with SMAD 4 and translocate to the nucleus. Reverse transcriptase-polymerase chain reaction showed that SMADs 1, 2 and 4 are expressed in pancreatic islets. Immunostaining revealed that SMAD 1 and 4 predominantly were expressed by islet insulin and glucagon cells. Since SMAD 1 is known to transduce signals from receptors binding bone morphogenetic protein (BMP) these results indicate a previously unknown role of BMP-like ligands in islet function.

AB - Members of the TGF-beta superfamily of cytokines have been implicated in pancreatic cancer, pancreatitis and in regulation and differentiation of pancreatic endocrine and exocrine cells. Different TGF-beta members signal through phosphorylation of different signal transduction proteins, which eventually form oligomers with SMAD 4 and translocate to the nucleus. Reverse transcriptase-polymerase chain reaction showed that SMADs 1, 2 and 4 are expressed in pancreatic islets. Immunostaining revealed that SMAD 1 and 4 predominantly were expressed by islet insulin and glucagon cells. Since SMAD 1 is known to transduce signals from receptors binding bone morphogenetic protein (BMP) these results indicate a previously unknown role of BMP-like ligands in islet function.

KW - Animals

KW - Animals, Newborn

KW - DNA-Binding Proteins

KW - Fluorescein-5-isothiocyanate

KW - Fluorescent Antibody Technique

KW - Gene Expression

KW - Immunohistochemistry

KW - Pancreas

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Signal Transduction

KW - Smad Proteins

KW - Smad1 Protein

KW - Smad2 Protein

KW - Smad4 Protein

KW - Trans-Activators

U2 - 10.1007/s004180100316

DO - 10.1007/s004180100316

M3 - Journal article

C2 - 11685556

SN - 0948-6143

VL - 116

SP - 263

EP - 267

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

IS - 3

ER -

Expression of SMAD signal transduction molecules in the pancreas

Abstract

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