Expression of protein-tyrosine phosphatases in the major insulin target tissues

K Norris; F Norris; D H Kono; H Vestergaard; O Pedersen; A N Theofilopoulos; N P Møller

Expression of protein-tyrosine phosphatases in the major insulin target tissues

K Norris, F Norris, D H Kono, H Vestergaard, O Pedersen, A N Theofilopoulos, N P Møller

51 Citationer (Scopus)

Abstract

Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	415
Udgave nummer	3
Sider (fra-til)	243-8
Antal sider	6
ISSN	0014-5793
Status	Udgivet - 6 okt. 1997

Citationsformater

@article{894e55c944334e269bc2d96326ba9d41,

title = "Expression of protein-tyrosine phosphatases in the major insulin target tissues",

abstract = "Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.",

keywords = "Adipose Tissue, Blotting, Northern, DNA Primers, Endothelium, Vascular, Gene Expression Regulation, Enzymologic, Humans, Isoenzymes, Muscle, Skeletal, Placenta, Polymerase Chain Reaction, Protein Tyrosine Phosphatases, RNA Probes, RNA, Messenger, Receptor, Insulin, Receptor-Like Protein Tyrosine Phosphatases, Class 4, Receptors, Cell Surface, Ribonucleases, Signal Transduction",

author = "K Norris and F Norris and Kono, {D H} and H Vestergaard and O Pedersen and Theofilopoulos, {A N} and M{\o}ller, {N P}",

year = "1997",

month = oct,

day = "6",

language = "English",

volume = "415",

pages = "243--8",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "3",

}

TY - JOUR

T1 - Expression of protein-tyrosine phosphatases in the major insulin target tissues

AU - Norris, K

AU - Norris, F

AU - Kono, D H

AU - Vestergaard, H

AU - Pedersen, O

AU - Theofilopoulos, A N

AU - Møller, N P

PY - 1997/10/6

Y1 - 1997/10/6

N2 - Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.

AB - Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.

KW - Adipose Tissue

KW - Blotting, Northern

KW - DNA Primers

KW - Endothelium, Vascular

KW - Gene Expression Regulation, Enzymologic

KW - Humans

KW - Isoenzymes

KW - Muscle, Skeletal

KW - Placenta

KW - Polymerase Chain Reaction

KW - Protein Tyrosine Phosphatases

KW - RNA Probes

KW - RNA, Messenger

KW - Receptor, Insulin

KW - Receptor-Like Protein Tyrosine Phosphatases, Class 4

KW - Receptors, Cell Surface

KW - Ribonucleases

KW - Signal Transduction

M3 - Journal article

C2 - 9357975

SN - 0014-5793

VL - 415

SP - 243

EP - 248

JO - F E B S Letters

JF - F E B S Letters

IS - 3

ER -

Expression of protein-tyrosine phosphatases in the major insulin target tissues

Abstract

Fingeraftryk

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