Expression of matrix metalloproteinase-9 and -12 in porcine lung infections

Camilla Vibeke Sichlau Bruun; Páll S. Leifsson; Louise Kruse Johansen; Henrik Michael Elvang Jensen; J. Nielsen; Merete Fredholm

doi:10.1016/j.jcpa.2011.05.005

Expression of matrix metalloproteinase-9 and -12 in porcine lung infections

Camilla Vibeke Sichlau Bruun, Páll S. Leifsson, Louise Kruse Johansen, Henrik Michael Elvang Jensen, J. Nielsen, Merete Fredholm

6 Citationer (Scopus)

Abstract

Matrix metalloproteinases (MMPs) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. During the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine MMPs are limited. In the present study species-specific antibodies were produced to investigate the expression of MMP-9 and MMP-12 immunohistochemically in lungs from pigs infected with Actinobacillus pleuropneumoniae, Pasteurella multocida and Staphylococcus aureus. The immunolabelling of lung tissues (one infected and one control pig representing each infection) was evaluated for cellular distribution and intensity, which was scored semiquantitatively. When compared with healthy, non-infected controls, the expression of both MMP-9 and MMP-12 was higher in infected lungs. The highest expressions were seen in the alveolar epithelium (MMP-9) and alveolar macrophages (MMP-12). These results are in accordance with studies of human lungs.

Originalsprog	Engelsk
Tidsskrift	Journal of Comparative Pathology
Vol/bind	146
Udgave nummer	2-3
Sider (fra-til)	253-257
Antal sider	5
ISSN	0021-9975
DOI	https://doi.org/10.1016/j.jcpa.2011.05.005
Status	Udgivet - feb. 2012

Emneord

Det tidligere LIFE
immunohistochemistry
MMP-9
MMP-12
pig

Adgang til dokumentet

10.1016/j.jcpa.2011.05.005

Citationsformater

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title = "Expression of matrix metalloproteinase-9 and -12 in porcine lung infections",

abstract = "Matrix metalloproteinases (MMPs) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. During the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine MMPs are limited. In the present study species-specific antibodies were produced to investigate the expression of MMP-9 and MMP-12 immunohistochemically in lungs from pigs infected with Actinobacillus pleuropneumoniae, Pasteurella multocida and Staphylococcus aureus. The immunolabelling of lung tissues (one infected and one control pig representing each infection) was evaluated for cellular distribution and intensity, which was scored semiquantitatively. When compared with healthy, non-infected controls, the expression of both MMP-9 and MMP-12 was higher in infected lungs. The highest expressions were seen in the alveolar epithelium (MMP-9) and alveolar macrophages (MMP-12). These results are in accordance with studies of human lungs.",

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author = "Bruun, {Camilla Vibeke Sichlau} and Leifsson, {P{\'a}ll S.} and Johansen, {Louise Kruse} and Jensen, {Henrik Michael Elvang} and J. Nielsen and Merete Fredholm",

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T1 - Expression of matrix metalloproteinase-9 and -12 in porcine lung infections

AU - Bruun, Camilla Vibeke Sichlau

AU - Leifsson, Páll S.

AU - Johansen, Louise Kruse

AU - Jensen, Henrik Michael Elvang

AU - Nielsen, J.

AU - Fredholm, Merete

PY - 2012/2

Y1 - 2012/2

N2 - Matrix metalloproteinases (MMPs) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. During the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine MMPs are limited. In the present study species-specific antibodies were produced to investigate the expression of MMP-9 and MMP-12 immunohistochemically in lungs from pigs infected with Actinobacillus pleuropneumoniae, Pasteurella multocida and Staphylococcus aureus. The immunolabelling of lung tissues (one infected and one control pig representing each infection) was evaluated for cellular distribution and intensity, which was scored semiquantitatively. When compared with healthy, non-infected controls, the expression of both MMP-9 and MMP-12 was higher in infected lungs. The highest expressions were seen in the alveolar epithelium (MMP-9) and alveolar macrophages (MMP-12). These results are in accordance with studies of human lungs.

AB - Matrix metalloproteinases (MMPs) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. During the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine MMPs are limited. In the present study species-specific antibodies were produced to investigate the expression of MMP-9 and MMP-12 immunohistochemically in lungs from pigs infected with Actinobacillus pleuropneumoniae, Pasteurella multocida and Staphylococcus aureus. The immunolabelling of lung tissues (one infected and one control pig representing each infection) was evaluated for cellular distribution and intensity, which was scored semiquantitatively. When compared with healthy, non-infected controls, the expression of both MMP-9 and MMP-12 was higher in infected lungs. The highest expressions were seen in the alveolar epithelium (MMP-9) and alveolar macrophages (MMP-12). These results are in accordance with studies of human lungs.

KW - Former LIFE faculty

KW - immunohistochemistry

KW - MMP-9

KW - MMP-12

KW - pig

U2 - 10.1016/j.jcpa.2011.05.005

DO - 10.1016/j.jcpa.2011.05.005

M3 - Journal article

SN - 0021-9975

VL - 146

SP - 253

EP - 257

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

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ER -