Expression of calcium channel Ca_V1.3 in cat spinal cord: light and electron microscopic immunohistochemical study

TY - JOUR

T1 - Expression of calcium channel CaV1.3 in cat spinal cord: light and electron microscopic immunohistochemical study

AU - Zhang, Mengliang

AU - Møller, Morten

AU - Broman, Jonas

AU - Sukiasyan, Natalya

AU - Wienecke, Jacob

AU - Hultborn, Hans

N1 - Keywords: Animals; Calcium Channels, L-Type; Cats; Cell Membrane; Dendrites; Endoplasmic Reticulum, Rough; Immunohistochemistry; Male; Microscopy, Immunoelectron; Neurons; Protein Subunits; Spinal Cord

PY - 2008

Y1 - 2008

N2 - In spinal neurons, plateau potentials serve to amplify neuronal input signals. To a large extent, the underlying persistent inward current is mediated by a subtype of the L-type calcium channel (Ca(V)1.3). In the present investigation, we have studied its distribution and cellular localization in the cat spinal cord by light and electron microscopic immunohistochemistry. The results show that Ca(V)1.3-like immunoreactivity is widely distributed in all segments of the spinal cord but that the distribution in the different laminae of the spinal gray matter varies, with the highest density of labeled neurons in lamina IX and the lowest in lamina II. The labeling intensity was highest in neuronal somata, but a certain length of the proximal dendrite was also labeled. Some neuronal groups exhibited a particularly dense labeling; these include the lateral motoneuronal group in the cervical and the lumbar enlargements and the phrenic nucleus in cervical, Clarke's nucleus in lower thoracic and upper lumbar, and Onuf's nucleus in upper sacral segments. At the ultrastructural level, Ca(V)1.3-immunoreactive products were found in neuronal somata and dendrites of different sizes. In the soma, they were predominantly associated with the rough endoplasmic reticulum but some also with the plasma membrane. In dendrites, they were associated with both intracellular organelles, including microtubules and microchondria, and the plasma membrane. These results indicate that significant proportions of the neurons in cat spinal cord, including projection neurons, interneurons, and motoneurons, are endowed with ion channels that subserve persistent inward currents and act to amplify synaptic input signals.

AB - In spinal neurons, plateau potentials serve to amplify neuronal input signals. To a large extent, the underlying persistent inward current is mediated by a subtype of the L-type calcium channel (Ca(V)1.3). In the present investigation, we have studied its distribution and cellular localization in the cat spinal cord by light and electron microscopic immunohistochemistry. The results show that Ca(V)1.3-like immunoreactivity is widely distributed in all segments of the spinal cord but that the distribution in the different laminae of the spinal gray matter varies, with the highest density of labeled neurons in lamina IX and the lowest in lamina II. The labeling intensity was highest in neuronal somata, but a certain length of the proximal dendrite was also labeled. Some neuronal groups exhibited a particularly dense labeling; these include the lateral motoneuronal group in the cervical and the lumbar enlargements and the phrenic nucleus in cervical, Clarke's nucleus in lower thoracic and upper lumbar, and Onuf's nucleus in upper sacral segments. At the ultrastructural level, Ca(V)1.3-immunoreactive products were found in neuronal somata and dendrites of different sizes. In the soma, they were predominantly associated with the rough endoplasmic reticulum but some also with the plasma membrane. In dendrites, they were associated with both intracellular organelles, including microtubules and microchondria, and the plasma membrane. These results indicate that significant proportions of the neurons in cat spinal cord, including projection neurons, interneurons, and motoneurons, are endowed with ion channels that subserve persistent inward currents and act to amplify synaptic input signals.

U2 - 10.1002/cne.21595

DO - 10.1002/cne.21595

M3 - Journal article

C2 - 18095323

SN - 0021-9967

VL - 507

SP - 1109

EP - 1127

JO - The Journal of Comparative Neurology

JF - The Journal of Comparative Neurology

IS - 1

ER -