Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

TY - JOUR

T1 - Expression of Bax in yeast affects not only the mitochondria but also vacuolar integrity and intracellular protein traffic

AU - Dimitrova, Irina

AU - Toby, Garabet G

AU - Tili, Esmerina

AU - Strich, Randy

AU - Kampranis, Sotirios C

AU - Makris, Antonios M

PY - 2004/5/21

Y1 - 2004/5/21

N2 - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

AB - Bax-induced lethality in yeast is accompanied by morphological changes in mitochondria, giving rise to a reduced number of swollen tubules. Although these changes are completely abolished upon coexpression of the Bax inhibitor, Bcl-2, coexpression of Bax with Bax inhibiting-glutathione S-transferase (BI-GST) leads to aggregation, but not fusion of the mitochondria. In addition, Bax affects the integrity of yeast vacuoles, resulting in the disintegration and eventual loss of the organelles, and the disruption of intracellular protein traffic. While Bcl-2 coexpression only partially corrects this phenotype, coexpression of BI-GST fully restores the organelles, indicating a different mode of protection exerted by Bcl-2 and BI-GST.

KW - Cell Survival

KW - Gene Expression

KW - Glutathione Transferase

KW - Green Fluorescent Proteins

KW - Intracellular Membranes

KW - Luminescent Proteins

KW - Microscopy, Fluorescence

KW - Mitochondria

KW - Protein Transport

KW - Proto-Oncogene Proteins

KW - Proto-Oncogene Proteins c-bcl-2

KW - Reactive Oxygen Species

KW - Recombinant Proteins

KW - Vacuoles

KW - Yeasts

KW - bcl-2-Associated X Protein

U2 - 10.1016/j.febslet.2004.04.012

DO - 10.1016/j.febslet.2004.04.012

M3 - Journal article

C2 - 15147876

SN - 0014-5793

VL - 566

SP - 100

EP - 104

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -