Exploring the complex response to linuron of bacterial communities from biopurification systems by means of cultivation-independent methods

TY - JOUR

T1 - Exploring the complex response to linuron of bacterial communities from biopurification systems by means of cultivation-independent methods

AU - Dealtry, Simone

AU - Nour, Eman H.

AU - Holmsgaard, Peter Nikolai

AU - Ding, Guo Chun

AU - Weichelt, Viola

AU - Dunon, Vincent

AU - Heuer, Holger

AU - Hansen, Lars H.

AU - Sørensen, Søren Johannes

AU - Springael, Dirk

AU - Smalla, Kornelia

PY - 2016/1/7

Y1 - 2016/1/7

N2 - On-farm biopurification systems (BPSs) treat pesticide-contaminated wastewater at farms through biodegradation and sorption processes. However, information on the microbiota involved in pesticide removal in BPSs is scarce. Here we report on the response of BPS bacterial communities to the herbicide linuron (BPS+) compared with the control (BPS-) in a microcosm experiment. Both denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of 16S rRNA gene fragments amplified from community DNA indicated shifts in the bacterial community after linuron application. Responding populations belonged to taxa that were previously reported from linuron degrading consortia cultivated from soil (Hyphomicrobiaceae, Comamonadaceae, Micrococcaceae). In addition, numerous taxa with increased relative abundance were identified that were previously not associated with linuron degradation. The relative abundance of IncP-1 korB copies increased in response to linuron application. Amplicon pyrosequencing of IncP-1 trfA genes revealed a high IncP-1 plasmid diversity and suggested that populations carrying IncP-1ß plasmids increased in relative abundance. Transferable mercury resistance plasmids were exogenously captured from BPS+/BPS-, and in three transconjugants from BPS+ the gene hylA was detected. Our data suggest the existence of a multispecies linuron degrading bacterial food web and an involvement of IncP-1 plasmids in the adaptation of bacterial communities to pesticide pollution in BPSs.

AB - On-farm biopurification systems (BPSs) treat pesticide-contaminated wastewater at farms through biodegradation and sorption processes. However, information on the microbiota involved in pesticide removal in BPSs is scarce. Here we report on the response of BPS bacterial communities to the herbicide linuron (BPS+) compared with the control (BPS-) in a microcosm experiment. Both denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of 16S rRNA gene fragments amplified from community DNA indicated shifts in the bacterial community after linuron application. Responding populations belonged to taxa that were previously reported from linuron degrading consortia cultivated from soil (Hyphomicrobiaceae, Comamonadaceae, Micrococcaceae). In addition, numerous taxa with increased relative abundance were identified that were previously not associated with linuron degradation. The relative abundance of IncP-1 korB copies increased in response to linuron application. Amplicon pyrosequencing of IncP-1 trfA genes revealed a high IncP-1 plasmid diversity and suggested that populations carrying IncP-1ß plasmids increased in relative abundance. Transferable mercury resistance plasmids were exogenously captured from BPS+/BPS-, and in three transconjugants from BPS+ the gene hylA was detected. Our data suggest the existence of a multispecies linuron degrading bacterial food web and an involvement of IncP-1 plasmids in the adaptation of bacterial communities to pesticide pollution in BPSs.

KW - Degradation

KW - IncP-1

KW - Linuron

KW - Pesticide

U2 - 10.1093/femsec/fiv157

DO - 10.1093/femsec/fiv157

M3 - Journal article

C2 - 26705572

SN - 0168-6496

VL - 92

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

IS - 2

M1 - fiv157

ER -