Exploring folding pathways of single proteins using mechanical manipulation

Pétur Orri Heiðarsson, Ciro Cecconi

Abstract

Protein folding is still a major area of active research. Despite significant progress in understanding the underlying principles, we still cannot efficiently predict the folding mechanism for even a moderately sized protein. Proteins are generally thought to fold by diffusion over a three-dimensional energy landscape. Traditional bulk methods have proven to be very powerful in the study of the folding process but they often suffer from inherent ensemble averaging. Single molecule techniques open up new vistas for studying protein folding, allowing direct analysis of the distribution of events that characterize the heterogeneous folding process. Recently it has become possible to directly manipulate individual proteins using optical tweezers. Here we illustrate the experimental strategy and how this approach has provided a fresh perspective on the protein folding problem.

OriginalsprogEngelsk
TitelTheory and applications in computational chemistry : the first decade of the second millennium
Redaktører Enrico Clementi, Jean-Marie André, J. Andrew McCammon
Antal sider6
ForlagAmerican Institute of Physics
Publikationsdato2012
Sider201-206
ISBN (Trykt) 978-0-7354-1057-2
DOI
StatusUdgivet - 2012
BegivenhedInternational Congress TACC-2012 - Pavia, Italien
Varighed: 2 sep. 20127 sep. 2012

Konference

KonferenceInternational Congress TACC-2012
Land/OmrådeItalien
ByPavia
Periode02/09/201207/09/2012
NavnA I P Conference Proceedings
Vol/bind1456
ISSN1551-7616

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