TY - JOUR
T1 - Exercise-induced increase in maximal in vitro Na-K-ATPase activity in human skeletal muscle
AU - Juel, Carsten
AU - Nordsborg, Nikolai Baastrup
AU - Bangsbo, Jens
N1 - CURIS 2013 NEXS 091
PY - 2013/6/15
Y1 - 2013/6/15
N2 - The present study investigated whether maximal in vitro Na-K-ATPase activity in human skeletal muscle is changed with exercise and whether it was altered by acute hypoxia. Needle biopsies from 14 subjects were obtained from vastus lateralis before and after 4 min of intense muscle activity. In addition, six subjects exercised also in hypoxia (12.5% oxygen). The Na-K-ATPase assay revealed a 19% increase (P < 0.05) in maximal velocity (Vmax) for Na+-dependent Na-K-ATPase activity after exercise and a tendency (P < 0.1) toward a decrease in Km for Na+ (increased Na+ affinity) in both normoxia and hypoxia. In contrast, the in vitro Na-K-ATPase activity determined with the 3-O-MFPase technique was 11-32% lower after exercise in normoxia (P < 0.05) and hypoxia (P < 0.1). Based on the different results obtained with the Na-K-ATPase assay and the 3-OMFPase technique, it was suggested that the 3-O-MFPase method is insensitive to changes in Na-K-ATPase activity. To test this possibility, changes in Na-K-ATPase activity was induced by protein kinase C activation. The changes quantified with the Na-K-ATPase assay could not be detected with the 3-O-MFPase method. In addition, purines stimulated Na-K-ATPase activity in rat muscle membranes; these changes could not be detected with the 3-O-MFPase method. Therefore, the 3-O-MFPase technique is not sensitive to changes in Na+ sensitivity, and the method is not suited to detecting changes in Na-K-ATPase activity with exercise. In conclusion, muscle activity in humans induces an increased in vitro Na+-dependent Na-KATPase activity, which contributes to the upregulation of the Na-KATPase in association with exercise both in normoxia and hypoxia.
AB - The present study investigated whether maximal in vitro Na-K-ATPase activity in human skeletal muscle is changed with exercise and whether it was altered by acute hypoxia. Needle biopsies from 14 subjects were obtained from vastus lateralis before and after 4 min of intense muscle activity. In addition, six subjects exercised also in hypoxia (12.5% oxygen). The Na-K-ATPase assay revealed a 19% increase (P < 0.05) in maximal velocity (Vmax) for Na+-dependent Na-K-ATPase activity after exercise and a tendency (P < 0.1) toward a decrease in Km for Na+ (increased Na+ affinity) in both normoxia and hypoxia. In contrast, the in vitro Na-K-ATPase activity determined with the 3-O-MFPase technique was 11-32% lower after exercise in normoxia (P < 0.05) and hypoxia (P < 0.1). Based on the different results obtained with the Na-K-ATPase assay and the 3-OMFPase technique, it was suggested that the 3-O-MFPase method is insensitive to changes in Na-K-ATPase activity. To test this possibility, changes in Na-K-ATPase activity was induced by protein kinase C activation. The changes quantified with the Na-K-ATPase assay could not be detected with the 3-O-MFPase method. In addition, purines stimulated Na-K-ATPase activity in rat muscle membranes; these changes could not be detected with the 3-O-MFPase method. Therefore, the 3-O-MFPase technique is not sensitive to changes in Na+ sensitivity, and the method is not suited to detecting changes in Na-K-ATPase activity with exercise. In conclusion, muscle activity in humans induces an increased in vitro Na+-dependent Na-KATPase activity, which contributes to the upregulation of the Na-KATPase in association with exercise both in normoxia and hypoxia.
U2 - 10.1152/ajpregu.00591.2012
DO - 10.1152/ajpregu.00591.2012
M3 - Journal article
C2 - 23576618
SN - 0363-6119
VL - 304
SP - R1161-R1165
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 12
ER -