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Examination of six commonly used laboratory fixatives in HAB monitoring programs for their use in quantitative PCR based on Taqman probe technology
Lisa Kathleen Eckford-Soper,
Niels Daugbjerg
Marine Biological Section
10
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Keyphrases
Aging Effect
25%
Alexandrium Tamarense
25%
Amplification Efficiency
25%
Cq Value
25%
Danish Waters
25%
Dinoflagellate
25%
DNA Template
25%
Ethanol
25%
Fixative
100%
Formalin
25%
Genomic DNA (gDNA)
50%
Glutaraldehyde
25%
Karenia Mikimotoi
25%
Karlodinium Veneficum
25%
Molecular Methods
25%
Monitoring Program
100%
Paraformaldehyde
25%
Parvum
25%
PCR-based
100%
Polymerase Chain Reaction Amplification
50%
Preserved Samples
25%
Prymnesium Parvum
25%
Quantitative PCR
100%
Rapid Detection
25%
Reliable Detection
25%
Sodium Thiosulfate
25%
TaqMan Probe
100%
Immunology and Microbiology
Alexandrium
25%
Dinoflagellate
25%
DNA Template
25%
Haptophyta
25%
Karenia
25%
Prymnesium
25%
Real-Time Polymerase Chain Reaction
100%
Agricultural and Biological Sciences
Haptophyte
25%
Prymnesium
25%
Sodium Thiosulfate
25%