TY - GEN
T1 - Evaluation of Y-STR analyses of sperm cell negative vaginal samples
AU - Olofsson, Jill Katharina
AU - Mogensen, Helle Smidt
AU - Hjort, Benjamin Benn
AU - Morling, Niels
PY - 2011/12
Y1 - 2011/12
N2 - In some cases of sexual assault, no sperm cells are microscopically visible in vaginal samples. In this study, sperm cell negative vaginal samples from 110 sexual assault cases in Denmark were analysed for Y-STRs with the AmpFlSTR® Yfiler kit (Applied Biosystems - AB). Selected samples were purified and concentrated using the MinElute (Qiagen) procedure. Y-DNA was detected in 49 (45%) cases. Complete or partial Y-STR profiles were obtained in 32 (29%) cases. Use of MinElute (Qiagen) significantly increased the success of typing of Y-STRs. In the majority of the cases, the Y-STR profiles observed were not found in three reference populations. Thus, informative Y-STR results were obtained in a significant proportion of the cases.
AB - In some cases of sexual assault, no sperm cells are microscopically visible in vaginal samples. In this study, sperm cell negative vaginal samples from 110 sexual assault cases in Denmark were analysed for Y-STRs with the AmpFlSTR® Yfiler kit (Applied Biosystems - AB). Selected samples were purified and concentrated using the MinElute (Qiagen) procedure. Y-DNA was detected in 49 (45%) cases. Complete or partial Y-STR profiles were obtained in 32 (29%) cases. Use of MinElute (Qiagen) significantly increased the success of typing of Y-STRs. In the majority of the cases, the Y-STR profiles observed were not found in three reference populations. Thus, informative Y-STR results were obtained in a significant proportion of the cases.
U2 - 10.1016/j.fsigss.2011.08.071
DO - 10.1016/j.fsigss.2011.08.071
M3 - Conference article
SN - 1875-1768
VL - Elektronisk
JO - Forensic Science International: Genetic Supplement Series
JF - Forensic Science International: Genetic Supplement Series
ER -