Evaluation of Lama5 as a candidate for the mouse ragged (Ra) mutation.

TY - JOUR

T1 - Evaluation of Lama5 as a candidate for the mouse ragged (Ra) mutation.

AU - Durkin, M E

AU - Albrechtsen, R

AU - Chambers, D M

AU - Abbott, C M

AU - Wewer, U M

N1 - Keywords: Animals; Base Sequence; Chromosome Mapping; DNA; Female; Heterozygote; Homozygote; Laminin; Male; Mice; Mice, Inbred C3H; Mice, Mutant Strains; Molecular Sequence Data; Mutation

PY - 1998

Y1 - 1998

N2 - The laminin alpha5 chain is a component of the basement membranes of many developing and adult tissues. The mouse laminin alpha5 chain gene (Lama5) has been mapped close to the locus of the semidominant ragged (Ra) mutation on distal chromosome 2. The cause of the Ra mutation, which is usually lethal in the homozygous state, has not been determined. We have investigated whether a defect in Lama5 is responsible for the ragged mutation, using the RaJ strain. No differences in the level of the laminin alpha5 chain transcript were found in placental RNA from homozygous RaJ mutant embryos compared to normal littermates. Antiserum raised against a recombinant laminin alpha5 chain polypeptide stained the basement membranes of both normal and homozygous mutant embryos to a similar extent. More precise mapping of Lama5 on an interspecific Ra backcross indicated that Lama5 is proximal to the Ra locus. These results exclude Lama5 as a candidate gene for the Ra mutation.

AB - The laminin alpha5 chain is a component of the basement membranes of many developing and adult tissues. The mouse laminin alpha5 chain gene (Lama5) has been mapped close to the locus of the semidominant ragged (Ra) mutation on distal chromosome 2. The cause of the Ra mutation, which is usually lethal in the homozygous state, has not been determined. We have investigated whether a defect in Lama5 is responsible for the ragged mutation, using the RaJ strain. No differences in the level of the laminin alpha5 chain transcript were found in placental RNA from homozygous RaJ mutant embryos compared to normal littermates. Antiserum raised against a recombinant laminin alpha5 chain polypeptide stained the basement membranes of both normal and homozygous mutant embryos to a similar extent. More precise mapping of Lama5 on an interspecific Ra backcross indicated that Lama5 is proximal to the Ra locus. These results exclude Lama5 as a candidate gene for the Ra mutation.

U2 - 10.1006/bbrc.1998.9285

DO - 10.1006/bbrc.1998.9285

M3 - Journal article

C2 - 9735344

SN - 0006-291X

VL - 250

SP - 125

EP - 130

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -