TY - JOUR
T1 - Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production
AU - Lindedam, Jane
AU - Bruun, Sander
AU - Jørgensen, Henning
AU - Decker, Stephen R.
AU - Turner, Geoffrey B.
AU - DeMartini, Jaclyn D.
AU - Wyman, Charles E.
AU - Felby, Claus
PY - 2014
Y1 - 2014
N2 - We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R2=0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2=0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.
AB - We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R2=0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2=0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.
KW - Genetic selection
KW - High throughput screening
KW - Lignocellulosic ethanol
KW - Microscale pretreatment
KW - Wheat straw
U2 - 10.1016/j.biombioe.2014.03.006
DO - 10.1016/j.biombioe.2014.03.006
M3 - Journal article
AN - SCOPUS:84901640759
SN - 0961-9534
VL - 66
SP - 261
EP - 267
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
ER -
