Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis

TY - JOUR

T1 - Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis

AU - Sørensen, Morten Draeby

AU - Gonzalez Dosal, Regina

AU - Jensen, Kim Bak

AU - Christensen, Britta

AU - Kølvraa, Steen

AU - Jensen, Uffe Birk

PY - 2009

Y1 - 2009

N2 - Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising epsilon-Hb, one was chosen for further characterization, DAb1. DAb1 binds to epsilon-Hb both in Western blots and immunocytochemistry. Several epsilon-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express epsilon-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.

AB - Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising epsilon-Hb, one was chosen for further characterization, DAb1. DAb1 binds to epsilon-Hb both in Western blots and immunocytochemistry. Several epsilon-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express epsilon-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.

KW - Antibodies/chemistry

KW - Blotting, Western

KW - Erythroblasts/cytology

KW - Female

KW - Fetal Blood/cytology

KW - Flow Cytometry

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - K562 Cells

KW - Peptide Library

KW - Pregnancy

KW - Prenatal Diagnosis/methods

KW - Recombinant Proteins/chemistry

KW - Sensitivity and Specificity

KW - epsilon-Globins/analysis

U2 - 10.1155/2009/659219

DO - 10.1155/2009/659219

M3 - Journal article

C2 - 19636421

SN - 1110-7243

VL - 2009

SP - 659219

JO - Journal of Biomedicine and Biotechnology

JF - Journal of Biomedicine and Biotechnology

ER -