TY - JOUR
T1 - EPR spin trapping of protein radicals
AU - Davies, Michael Jonathan
AU - Hawkins, Clare Louise
PY - 2004
Y1 - 2004
N2 - Electron paramagnetic resonance (EPR) spin trapping was originally developed to aid the detection of low-molecular-mass radicals formed in chemical systems. It has subsequently found widespread use in biology and medicine for the direct detection of radical species formed during oxidative stress and via enzymatic reactions. Over the last 15 years this technique has also found increasing use in detecting and identifying radicals formed on biological macromolecules as a result of either radical reactions or enzymatic processes. Though the EPR signals that result from the trapping of large, slowly tumbling radicals are often broad and relatively poor in distinctive features, a number of techniques have been developed that allow a wealth of information to be obtained about the nature, site, and reactions of such radicals. This article summarizes recent developments in this area and reviews selected examples of radical formation on proteins.
AB - Electron paramagnetic resonance (EPR) spin trapping was originally developed to aid the detection of low-molecular-mass radicals formed in chemical systems. It has subsequently found widespread use in biology and medicine for the direct detection of radical species formed during oxidative stress and via enzymatic reactions. Over the last 15 years this technique has also found increasing use in detecting and identifying radicals formed on biological macromolecules as a result of either radical reactions or enzymatic processes. Though the EPR signals that result from the trapping of large, slowly tumbling radicals are often broad and relatively poor in distinctive features, a number of techniques have been developed that allow a wealth of information to be obtained about the nature, site, and reactions of such radicals. This article summarizes recent developments in this area and reviews selected examples of radical formation on proteins.
KW - Artifacts
KW - Electron Spin Resonance Spectroscopy
KW - Free Radicals
KW - Oxidation-Reduction
KW - Proteins
KW - Spin Trapping
U2 - 10.1016/j.freeradbiomed.2003.12.013
DO - 10.1016/j.freeradbiomed.2003.12.013
M3 - Journal article
C2 - 15082061
SN - 0891-5849
VL - 36
SP - 1072
EP - 1086
JO - Free Radical Biology & Medicine
JF - Free Radical Biology & Medicine
IS - 9
ER -