Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis

Anne-Christine Bay-Jensen; Qi Liu; Inger Byrjalsen; Yi Li; Jianxia Wang; Christian Pedersen; Diana J Leeming; Erik B Dam; Qinlong Zheng; Per Qvist; Morten A Karsdal

doi:10.1016/j.clinbiochem.2011.01.001

Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis

Anne-Christine Bay-Jensen, Qi Liu, Inger Byrjalsen, Yi Li, Jianxia Wang, Christian Pedersen, Diana J Leeming, Erik B Dam, Qinlong Zheng, Per Qvist, Morten A Karsdal

Datalogisk Institut

100 Citationer (Scopus)

Abstract

Objectives: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. Methods: A collagen type II specific neoepitope, CIIM, was identified (RDGAAG¹⁰⁵³) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. Results: The ELISAs showed good technical performance; CV%, < 13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P< 0.05) in those individuals with mild or severe OA than in those with no OA. Conclusion: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.

Originalsprog	Engelsk
Tidsskrift	Clinical Biochemistry
Vol/bind	44
Udgave nummer	5-6
Sider (fra-til)	423-9
Antal sider	7
ISSN	0009-9120
DOI	https://doi.org/10.1016/j.clinbiochem.2011.01.001
Status	Udgivet - apr. 2011

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10.1016/j.clinbiochem.2011.01.001

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Bay-Jensen, A-C., Liu, Q., Byrjalsen, I., Li, Y., Wang, J., Pedersen, C., Leeming, D. J., Dam, E. B., Zheng, Q., Qvist, P., & Karsdal, M. A. (2011). Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis. Clinical Biochemistry, 44(5-6), 423-9. https://doi.org/10.1016/j.clinbiochem.2011.01.001

Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis. / Bay-Jensen, Anne-Christine; Liu, Qi; Byrjalsen, Inger et al.

I: Clinical Biochemistry, Bind 44, Nr. 5-6, 04.2011, s. 423-9.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Bay-Jensen, A-C, Liu, Q, Byrjalsen, I, Li, Y, Wang, J, Pedersen, C, Leeming, DJ, Dam, EB, Zheng, Q, Qvist, P & Karsdal, MA 2011, 'Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis', Clinical Biochemistry, bind 44, nr. 5-6, s. 423-9. https://doi.org/10.1016/j.clinbiochem.2011.01.001

@article{d302537ac45e4f25a76e49a8e7c28c1d,

title = "Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis",

abstract = "Objectives: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. Methods: A collagen type II specific neoepitope, CIIM, was identified (RDGAAG1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. Results: The ELISAs showed good technical performance; CV%, < 13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P< 0.05) in those individuals with mild or severe OA than in those with no OA. Conclusion: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.",

keywords = "Aged, Aged, 80 and over, Collagen Type II, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Metalloproteases, Middle Aged, Osteoarthritis, Journal Article",

author = "Anne-Christine Bay-Jensen and Qi Liu and Inger Byrjalsen and Yi Li and Jianxia Wang and Christian Pedersen and Leeming, {Diana J} and Dam, {Erik B} and Qinlong Zheng and Per Qvist and Karsdal, {Morten A}",

year = "2011",

month = apr,

doi = "10.1016/j.clinbiochem.2011.01.001",

language = "English",

volume = "44",

pages = "423--9",

journal = "Clinical Biochemistry",

issn = "0009-9120",

publisher = "Elsevier",

number = "5-6",

}

TY - JOUR

T1 - Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis

AU - Bay-Jensen, Anne-Christine

AU - Liu, Qi

AU - Byrjalsen, Inger

AU - Li, Yi

AU - Wang, Jianxia

AU - Pedersen, Christian

AU - Leeming, Diana J

AU - Dam, Erik B

AU - Zheng, Qinlong

AU - Qvist, Per

AU - Karsdal, Morten A

PY - 2011/4

Y1 - 2011/4

N2 - Objectives: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. Methods: A collagen type II specific neoepitope, CIIM, was identified (RDGAAG1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. Results: The ELISAs showed good technical performance; CV%, < 13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P< 0.05) in those individuals with mild or severe OA than in those with no OA. Conclusion: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.

AB - Objectives: In joint degenerative diseases, the collagens are degraded by matrix metalloproteinases and protein fragments are released to serum as potential biomarkers. Methods: A collagen type II specific neoepitope, CIIM, was identified (RDGAAG1053) by mass spectrometry. Two ELISAs against the neoepitope were developed. CIIM was measured in cartilage explants in the presence or absence of protease inhibitors. CIIM was measured in OA synovial fluid (n=51) and serum (n=156). Knee OA was graded by standard Kellgren-Lawrence (KL) score. Results: The ELISAs showed good technical performance; CV%, < 13%. CIIM release from cartilage explants was blocked by the MMP inhibitor. CIIM was detected in synovial fluid. Furthermore, serum CIIM levels were significantly higher (P< 0.05) in those individuals with mild or severe OA than in those with no OA. Conclusion: We developed a new biomarker for joint degenerative diseases, which we demonstrated was derived from MMP-degraded type II collagen.

KW - Aged

KW - Aged, 80 and over

KW - Collagen Type II

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Male

KW - Metalloproteases

KW - Middle Aged

KW - Osteoarthritis

KW - Journal Article

U2 - 10.1016/j.clinbiochem.2011.01.001

DO - 10.1016/j.clinbiochem.2011.01.001

M3 - Journal article

C2 - 21223960

SN - 0009-9120

VL - 44

SP - 423

EP - 429

JO - Clinical Biochemistry

JF - Clinical Biochemistry

IS - 5-6

ER -

Enzyme-linked immunosorbent assay (ELISAs) for metalloproteinase derived type II collagen neoepitope, CIIM--increased serum CIIM in subjects with severe radiographic osteoarthritis

Abstract

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