Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula

Lisa Adolfsson; Hugues Nziengui; Ilka N. Abreu; Jan Simura; Azeez Beebo; Andrei Herdean; Jila Aboalizadeh; Jitka Siroka; Thomas Moritz; Ondrej Novak; Karin Ljung; Benoit Schoefs; Cornelia Spetea

doi:10.1104/pp.16.01509

Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula

Lisa Adolfsson, Hugues Nziengui, Ilka N. Abreu, Jan Simura, Azeez Beebo, Andrei Herdean, Jila Aboalizadeh, Jitka Siroka, Thomas Moritz, Ondrej Novak, Karin Ljung, Benoit Schoefs, Cornelia Spetea

34 Citationer (Scopus)

Abstract

Arbuscular mycorrhizas (AM) are the most common symbiotic associations between a plant’s root compartment and fungi. They provide nutritional benefit (mostly inorganic phosphate [P_i ]), leading to improved growth, and nonnutritional benefits, including defense responses to environmental cues throughout the host plant, which, in return, delivers carbohydrates to the symbiont. However, how transcriptional and metabolic changes occurring in leaves of AM plants differ from those induced by P fertilization is poorly understood. We investigated systemic changes in the leaves of mycorrhized Medicago truncatula inⁱ conditions with no improved P_i status and compared them with those induced by high-P_i treatment in nonmycorrhized plants. Microarray-based genome-wide profiling indicated up-regulation by mycorrhization of genes involved in flavonoid, terpenoid, jasmonic acid (JA), and abscisic acid (ABA) biosynthesis as well as enhanced expression of MYC2, the master regulator of JA-dependent responses. Accordingly, total anthocyanins and flavonoids increased, and most flavonoid species were enriched in AM leaves. Both the AM and P_i treatments corepressed iron homeostasis genes, resulting in lower levels of available iron in leaves. In addition, higher levels of cytokinins were found in leaves of AM- and P_i-treated plants, whereas the level of ABA was increased specifically in AM leaves. Foliar treatment of nonmycorrhized plants with either ABA or JA induced the up-regulation of MYC2, but only JA also induced the up-regulation of flavonoid and terpenoid biosynthetic genes. Based on these results, we propose that mycorrhization and P_i fertilization share cytokinin-mediated improved shoot growth, whereas enhanced ABA biosynthesis and JA-regulated flavonoid and terpenoid biosynthesis in leaves are specific to mycorrhization.

Originalsprog	Engelsk
Tidsskrift	Plant Physiology
Vol/bind	175
Udgave nummer	1
Sider (fra-til)	392-411
Antal sider	20
ISSN	0032-0889
DOI	https://doi.org/10.1104/pp.16.01509
Status	Udgivet - sep. 2017
Udgivet eksternt	Ja

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10.1104/pp.16.01509

392.full.pdfForlagets udgivne version, 1,56 MB

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580739/

Citationsformater

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title = "Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula",

abstract = "Arbuscular mycorrhizas (AM) are the most common symbiotic associations between a plant{\textquoteright}s root compartment and fungi. They provide nutritional benefit (mostly inorganic phosphate [Pi ]), leading to improved growth, and nonnutritional benefits, including defense responses to environmental cues throughout the host plant, which, in return, delivers carbohydrates to the symbiont. However, how transcriptional and metabolic changes occurring in leaves of AM plants differ from those induced by P fertilization is poorly understood. We investigated systemic changes in the leaves of mycorrhized Medicago truncatula ini conditions with no improved Pi status and compared them with those induced by high-Pi treatment in nonmycorrhized plants. Microarray-based genome-wide profiling indicated up-regulation by mycorrhization of genes involved in flavonoid, terpenoid, jasmonic acid (JA), and abscisic acid (ABA) biosynthesis as well as enhanced expression of MYC2, the master regulator of JA-dependent responses. Accordingly, total anthocyanins and flavonoids increased, and most flavonoid species were enriched in AM leaves. Both the AM and Pi treatments corepressed iron homeostasis genes, resulting in lower levels of available iron in leaves. In addition, higher levels of cytokinins were found in leaves of AM- and Pi-treated plants, whereas the level of ABA was increased specifically in AM leaves. Foliar treatment of nonmycorrhized plants with either ABA or JA induced the up-regulation of MYC2, but only JA also induced the up-regulation of flavonoid and terpenoid biosynthetic genes. Based on these results, we propose that mycorrhization and Pi fertilization share cytokinin-mediated improved shoot growth, whereas enhanced ABA biosynthesis and JA-regulated flavonoid and terpenoid biosynthesis in leaves are specific to mycorrhization.",

author = "Lisa Adolfsson and Hugues Nziengui and Abreu, {Ilka N.} and Jan Simura and Azeez Beebo and Andrei Herdean and Jila Aboalizadeh and Jitka Siroka and Thomas Moritz and Ondrej Novak and Karin Ljung and Benoit Schoefs and Cornelia Spetea",

year = "2017",

month = sep,

doi = "10.1104/pp.16.01509",

language = "English",

volume = "175",

pages = "392--411",

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T1 - Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula

AU - Adolfsson, Lisa

AU - Nziengui, Hugues

AU - Abreu, Ilka N.

AU - Simura, Jan

AU - Beebo, Azeez

AU - Herdean, Andrei

AU - Aboalizadeh, Jila

AU - Siroka, Jitka

AU - Moritz, Thomas

AU - Novak, Ondrej

AU - Ljung, Karin

AU - Schoefs, Benoit

AU - Spetea, Cornelia

PY - 2017/9

Y1 - 2017/9

N2 - Arbuscular mycorrhizas (AM) are the most common symbiotic associations between a plant’s root compartment and fungi. They provide nutritional benefit (mostly inorganic phosphate [Pi ]), leading to improved growth, and nonnutritional benefits, including defense responses to environmental cues throughout the host plant, which, in return, delivers carbohydrates to the symbiont. However, how transcriptional and metabolic changes occurring in leaves of AM plants differ from those induced by P fertilization is poorly understood. We investigated systemic changes in the leaves of mycorrhized Medicago truncatula ini conditions with no improved Pi status and compared them with those induced by high-Pi treatment in nonmycorrhized plants. Microarray-based genome-wide profiling indicated up-regulation by mycorrhization of genes involved in flavonoid, terpenoid, jasmonic acid (JA), and abscisic acid (ABA) biosynthesis as well as enhanced expression of MYC2, the master regulator of JA-dependent responses. Accordingly, total anthocyanins and flavonoids increased, and most flavonoid species were enriched in AM leaves. Both the AM and Pi treatments corepressed iron homeostasis genes, resulting in lower levels of available iron in leaves. In addition, higher levels of cytokinins were found in leaves of AM- and Pi-treated plants, whereas the level of ABA was increased specifically in AM leaves. Foliar treatment of nonmycorrhized plants with either ABA or JA induced the up-regulation of MYC2, but only JA also induced the up-regulation of flavonoid and terpenoid biosynthetic genes. Based on these results, we propose that mycorrhization and Pi fertilization share cytokinin-mediated improved shoot growth, whereas enhanced ABA biosynthesis and JA-regulated flavonoid and terpenoid biosynthesis in leaves are specific to mycorrhization.

AB - Arbuscular mycorrhizas (AM) are the most common symbiotic associations between a plant’s root compartment and fungi. They provide nutritional benefit (mostly inorganic phosphate [Pi ]), leading to improved growth, and nonnutritional benefits, including defense responses to environmental cues throughout the host plant, which, in return, delivers carbohydrates to the symbiont. However, how transcriptional and metabolic changes occurring in leaves of AM plants differ from those induced by P fertilization is poorly understood. We investigated systemic changes in the leaves of mycorrhized Medicago truncatula ini conditions with no improved Pi status and compared them with those induced by high-Pi treatment in nonmycorrhized plants. Microarray-based genome-wide profiling indicated up-regulation by mycorrhization of genes involved in flavonoid, terpenoid, jasmonic acid (JA), and abscisic acid (ABA) biosynthesis as well as enhanced expression of MYC2, the master regulator of JA-dependent responses. Accordingly, total anthocyanins and flavonoids increased, and most flavonoid species were enriched in AM leaves. Both the AM and Pi treatments corepressed iron homeostasis genes, resulting in lower levels of available iron in leaves. In addition, higher levels of cytokinins were found in leaves of AM- and Pi-treated plants, whereas the level of ABA was increased specifically in AM leaves. Foliar treatment of nonmycorrhized plants with either ABA or JA induced the up-regulation of MYC2, but only JA also induced the up-regulation of flavonoid and terpenoid biosynthetic genes. Based on these results, we propose that mycorrhization and Pi fertilization share cytokinin-mediated improved shoot growth, whereas enhanced ABA biosynthesis and JA-regulated flavonoid and terpenoid biosynthesis in leaves are specific to mycorrhization.

U2 - 10.1104/pp.16.01509

DO - 10.1104/pp.16.01509

M3 - Journal article

C2 - 28698354

SN - 0032-0889

VL - 175

SP - 392

EP - 411

JO - Plant Physiology

JF - Plant Physiology

IS - 1

ER -

Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula

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