Enhanced bacterial mutualism through an evolved biofilm phenotype

Henriette Lyng Røder; Jakob Herschend; Jakob Russel; Michala F. Andersen; Jonas Stenløkke Madsen; Søren Johannes Sørensen; Mette Burmølle

doi:10.1038/s41396-018-0165-2

Enhanced bacterial mutualism through an evolved biofilm phenotype

Henriette Lyng Røder, Jakob Herschend, Jakob Russel, Michala F. Andersen, Jonas Stenløkke Madsen, Søren Johannes Sørensen, Mette Burmølle

Microbiology

11 Citationer (Scopus)

Abstract

Microbial communities primarily consist of multiple species that affect one another's fitness both directly and indirectly. This study showed that the cocultivation of Paenibacillus amylolyticus and Xanthomonas retroflexus exhibited facultative mutualistic interactions in a static environment, during the course of which a new adapted phenotypic variant of X. retroflexus appeared. Although the emergence of this variant was not directly linked to the presence of P. amylolyticus, its establishment in the coculture enhanced the productivity of both species due to mutations that stimulated biofilm formation. The mutations were detected in genes encoding a diguanylate cyclase predicted to synthesise cyclic-di-GMP. Examinations of the biofilm formed in cocultures of P. amylolyticus and the new variant of X. retroflexus revealed a distinct spatial organisation: P. amylolyticus only resided in biofilms in association with X. retroflexus and occupied the outer layers. The X. retroflexus variant therefore facilitated increased P. amylolyticus growth as it produced more biofilm biomass. The increase in X. retroflexus biomass was thus not at the expense of P. amylolyticus, demonstrating that interspecies interactions can shape diversification in a mutualistic coculture and reinforce these interactions, ultimately resulting in enhanced communal performance.

Originalsprog	Engelsk
Tidsskrift	I S M E Journal
Vol/bind	12
Sider (fra-til)	2608-2618
Antal sider	11
ISSN	1751-7362
DOI	https://doi.org/10.1038/s41396-018-0165-2
Status	Udgivet - 1 nov. 2018

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10.1038/s41396-018-0165-2

s41396-018-0165-2.pdfForlagets udgivne version, 1,38 MB

Citationsformater

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title = "Enhanced bacterial mutualism through an evolved biofilm phenotype",

abstract = "Microbial communities primarily consist of multiple species that affect one another's fitness both directly and indirectly. This study showed that the cocultivation of Paenibacillus amylolyticus and Xanthomonas retroflexus exhibited facultative mutualistic interactions in a static environment, during the course of which a new adapted phenotypic variant of X. retroflexus appeared. Although the emergence of this variant was not directly linked to the presence of P. amylolyticus, its establishment in the coculture enhanced the productivity of both species due to mutations that stimulated biofilm formation. The mutations were detected in genes encoding a diguanylate cyclase predicted to synthesise cyclic-di-GMP. Examinations of the biofilm formed in cocultures of P. amylolyticus and the new variant of X. retroflexus revealed a distinct spatial organisation: P. amylolyticus only resided in biofilms in association with X. retroflexus and occupied the outer layers. The X. retroflexus variant therefore facilitated increased P. amylolyticus growth as it produced more biofilm biomass. The increase in X. retroflexus biomass was thus not at the expense of P. amylolyticus, demonstrating that interspecies interactions can shape diversification in a mutualistic coculture and reinforce these interactions, ultimately resulting in enhanced communal performance.",

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T1 - Enhanced bacterial mutualism through an evolved biofilm phenotype

AU - Røder, Henriette Lyng

AU - Herschend, Jakob

AU - Russel, Jakob

AU - Andersen, Michala F.

AU - Madsen, Jonas Stenløkke

AU - Sørensen, Søren Johannes

AU - Burmølle, Mette

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Microbial communities primarily consist of multiple species that affect one another's fitness both directly and indirectly. This study showed that the cocultivation of Paenibacillus amylolyticus and Xanthomonas retroflexus exhibited facultative mutualistic interactions in a static environment, during the course of which a new adapted phenotypic variant of X. retroflexus appeared. Although the emergence of this variant was not directly linked to the presence of P. amylolyticus, its establishment in the coculture enhanced the productivity of both species due to mutations that stimulated biofilm formation. The mutations were detected in genes encoding a diguanylate cyclase predicted to synthesise cyclic-di-GMP. Examinations of the biofilm formed in cocultures of P. amylolyticus and the new variant of X. retroflexus revealed a distinct spatial organisation: P. amylolyticus only resided in biofilms in association with X. retroflexus and occupied the outer layers. The X. retroflexus variant therefore facilitated increased P. amylolyticus growth as it produced more biofilm biomass. The increase in X. retroflexus biomass was thus not at the expense of P. amylolyticus, demonstrating that interspecies interactions can shape diversification in a mutualistic coculture and reinforce these interactions, ultimately resulting in enhanced communal performance.

AB - Microbial communities primarily consist of multiple species that affect one another's fitness both directly and indirectly. This study showed that the cocultivation of Paenibacillus amylolyticus and Xanthomonas retroflexus exhibited facultative mutualistic interactions in a static environment, during the course of which a new adapted phenotypic variant of X. retroflexus appeared. Although the emergence of this variant was not directly linked to the presence of P. amylolyticus, its establishment in the coculture enhanced the productivity of both species due to mutations that stimulated biofilm formation. The mutations were detected in genes encoding a diguanylate cyclase predicted to synthesise cyclic-di-GMP. Examinations of the biofilm formed in cocultures of P. amylolyticus and the new variant of X. retroflexus revealed a distinct spatial organisation: P. amylolyticus only resided in biofilms in association with X. retroflexus and occupied the outer layers. The X. retroflexus variant therefore facilitated increased P. amylolyticus growth as it produced more biofilm biomass. The increase in X. retroflexus biomass was thus not at the expense of P. amylolyticus, demonstrating that interspecies interactions can shape diversification in a mutualistic coculture and reinforce these interactions, ultimately resulting in enhanced communal performance.

U2 - 10.1038/s41396-018-0165-2

DO - 10.1038/s41396-018-0165-2

M3 - Journal article

C2 - 29977009

SN - 1751-7362

VL - 12

SP - 2608

EP - 2618

JO - I S M E Journal

JF - I S M E Journal

ER -

Enhanced bacterial mutualism through an evolved biofilm phenotype

Abstract

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