Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia

Ana R Inácio; Yawei Liu; Bettina H Clausen; Martina Svensson; Krzysztof Kucharz; Yiyi Yang; Totte Stankovich; Reza Khorooshi; Kate L Lambertsen; Shohreh Issazadeh-Navikas; Tomas Deierborg

doi:10.1186/s12974-015-0427-0

Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia

Ana R Inácio, Yawei Liu, Bettina H Clausen, Martina Svensson, Krzysztof Kucharz, Yiyi Yang, Totte Stankovich, Reza Khorooshi, Kate L Lambertsen, Shohreh Issazadeh-Navikas, Tomas Deierborg

Neuronal Signalling

25 Citationer (Scopus)

Abstract

BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.

METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.

RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.

CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.

Originalsprog	Engelsk
Artikelnummer	211
Tidsskrift	Journal of Neuroinflammation
Vol/bind	12
Sider (fra-til)	1-18
ISSN	1742-2094
DOI	https://doi.org/10.1186/s12974-015-0427-0
Status	Udgivet - 18 nov. 2015

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10.1186/s12974-015-0427-0Licens: CC BY

Citationsformater

Inácio, A. R., Liu, Y., Clausen, B. H., Svensson, M., Kucharz, K., Yang, Y., Stankovich, T., Khorooshi, R., Lambertsen, K. L., Issazadeh-Navikas, S., & Deierborg, T. (2015). Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia. Journal of Neuroinflammation, 12, 1-18. Artikel 211. https://doi.org/10.1186/s12974-015-0427-0

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title = "Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia",

abstract = "BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.",

keywords = "Animals, B-Lymphocytes, Brain, Brain Ischemia, Cytokines, Hand Strength, Infarction, Middle Cerebral Artery, Inflammation, Interferon-beta, Ischemic Attack, Transient, Leukocytes, Lymphocyte Count, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Postural Balance, Receptors, Interferon, Spleen, Stroke",

author = "In{\'a}cio, {Ana R} and Yawei Liu and Clausen, {Bettina H} and Martina Svensson and Krzysztof Kucharz and Yiyi Yang and Totte Stankovich and Reza Khorooshi and Lambertsen, {Kate L} and Shohreh Issazadeh-Navikas and Tomas Deierborg",

year = "2015",

month = nov,

day = "18",

doi = "10.1186/s12974-015-0427-0",

language = "English",

volume = "12",

pages = "1--18",

journal = "Journal of Neuroinflammation",

issn = "1742-2094",

publisher = "BioMed Central",

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TY - JOUR

T1 - Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia

AU - Inácio, Ana R

AU - Liu, Yawei

AU - Clausen, Bettina H

AU - Svensson, Martina

AU - Kucharz, Krzysztof

AU - Yang, Yiyi

AU - Stankovich, Totte

AU - Khorooshi, Reza

AU - Lambertsen, Kate L

AU - Issazadeh-Navikas, Shohreh

AU - Deierborg, Tomas

PY - 2015/11/18

Y1 - 2015/11/18

N2 - BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.

AB - BACKGROUND: Interferon (IFN)-β exerts anti-inflammatory effects, coupled to remarkable neurological improvements in multiple sclerosis, a neuroinflammatory condition of the central nervous system. Analogously, it has been hypothesized that IFN-β, by limiting inflammation, decreases neuronal death and promotes functional recovery after stroke. However, the core actions of endogenous IFN-β signaling in stroke are unclear.METHODS: To address this question, we used two clinically relevant models of focal cerebral ischemia, transient and permanent middle cerebral artery occlusion, and two genetically modified mouse lines, lacking either IFN-β or its receptor, the IFN-α/β receptor. Subsets of inflammatory and immune cells isolated from the brain, blood, and spleen were studied using flow cytometry. Sensorimotor deficits were assessed by a modified composite neuroscore, the rotating pole and grip strength tests, and cerebral infarct volumes were given by lack of neuronal nuclei immunoreactivity.RESULTS: Here, we report alterations in local and systemic inflammation in IFN-β knockout (IFN-βKO) mice over 8 days after induction of focal cerebral ischemia. Notably, IFN-βKO mice showed a higher number of infiltrating leukocytes in the brain 2 days after stroke. Concomitantly, in the blood of IFN-βKO mice, we found a higher percentage of total B cells but a similar percentage of mature and activated B cells, collectively indicating a higher proliferation rate. The additional differential regulation of circulating cytokines and splenic immune cell populations in wild-type and IFN-βKO mice further supports an important immunoregulatory function of IFN-β in stroke. Moreover, we observed a significant weight loss 2-3 days and a reduction in grip strength 2 days after stroke in the IFN-βKO group, while endogenous IFN-β signaling did not affect the infarct volume.CONCLUSIONS: We conclude that endogenous IFN-β signaling attenuates local inflammation, regulates peripheral immune cells, and, thereby, may contribute positively to stroke outcome.

KW - Animals

KW - B-Lymphocytes

KW - Brain

KW - Brain Ischemia

KW - Cytokines

KW - Hand Strength

KW - Infarction, Middle Cerebral Artery

KW - Inflammation

KW - Interferon-beta

KW - Ischemic Attack, Transient

KW - Leukocytes

KW - Lymphocyte Count

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Postural Balance

KW - Receptors, Interferon

KW - Spleen

KW - Stroke

U2 - 10.1186/s12974-015-0427-0

DO - 10.1186/s12974-015-0427-0

M3 - Journal article

C2 - 26581581

SN - 1742-2094

VL - 12

SP - 1

EP - 18

JO - Journal of Neuroinflammation

JF - Journal of Neuroinflammation

M1 - 211

ER -

Endogenous IFN-β signaling exerts anti-inflammatory actions in experimentally induced focal cerebral ischemia

Abstract

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