TY - JOUR
T1 - Vogesella mureinivorans sp. nov., a peptidoglycan-degrading bacterium from lake water
AU - Jørgensen, Niels O. G.
AU - Brandt, Kristian Koefoed
AU - Nybroe, Ole
AU - Hansen, Michael
PY - 2010/10
Y1 - 2010/10
N2 - A novel, non-pigmented, rod-shaped, Gram-negative strain was isolated from mesotrophic lake water in Zealand, Denmark. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium, designated strain 389T, indicated that the strain belonged to the genus Vogesella and formed a monophyletic group with Vogesella perlucida DS-28T (99.1% nucleotide similarity); it was less related to Vogesella indigofera ATCC 19706T (96.9% similarity) and Vogesella lacus LMG 24504T (96.8% similarity). Hybridization of DNA from strain 389T and V. perlucida demonstrated a reassociation of 50.6 ± 9.6 %. The DNA G+C content of strain 389 T was 61.2 mol%. The fatty acid profile of the strain differed from those of the other strains representing the genus Vogesella by a high content of C16 : 1ω7c and/or iso-C15 : 0 2-OH (71.6 %) and a lower content of C16 : 0. Strain 389T was capable of degrading peptidoglycan and had chitinase and lysozyme activities, possibly associated with the degradation of peptidoglycan, and had capacity for degradation of several other polymer compounds. Based on phenotypic and genotypic characteristics, strain 389T represents a novel species, for which we propose the name Vogesella mureinivorans sp. nov. The type strain is 389T (=DSM 21247T =LMG 25302T).
AB - A novel, non-pigmented, rod-shaped, Gram-negative strain was isolated from mesotrophic lake water in Zealand, Denmark. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium, designated strain 389T, indicated that the strain belonged to the genus Vogesella and formed a monophyletic group with Vogesella perlucida DS-28T (99.1% nucleotide similarity); it was less related to Vogesella indigofera ATCC 19706T (96.9% similarity) and Vogesella lacus LMG 24504T (96.8% similarity). Hybridization of DNA from strain 389T and V. perlucida demonstrated a reassociation of 50.6 ± 9.6 %. The DNA G+C content of strain 389 T was 61.2 mol%. The fatty acid profile of the strain differed from those of the other strains representing the genus Vogesella by a high content of C16 : 1ω7c and/or iso-C15 : 0 2-OH (71.6 %) and a lower content of C16 : 0. Strain 389T was capable of degrading peptidoglycan and had chitinase and lysozyme activities, possibly associated with the degradation of peptidoglycan, and had capacity for degradation of several other polymer compounds. Based on phenotypic and genotypic characteristics, strain 389T represents a novel species, for which we propose the name Vogesella mureinivorans sp. nov. The type strain is 389T (=DSM 21247T =LMG 25302T).
U2 - 10.1099/ijs.0.018630-0
DO - 10.1099/ijs.0.018630-0
M3 - Journal article
C2 - 19946047
SN - 1466-5026
VL - 60
SP - 2467
EP - 2472
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
ER -