Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

Henrik Christensen; Anders Miki Bojesen; Magne Bisgaard

doi:10.1099/ijs.0.026518-0

Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

Henrik Christensen, Anders Miki Bojesen, Magne Bisgaard

14 Citationer (Scopus)

Abstract

Strains T138021-75 ^T, Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75 ^T and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6%. These two strains showed 97.8% sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3% similarity with the type strain of Mannheimia varigena, but,97% similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9%). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9% gene sequence similarity) compared with the three other strains (T138021-75 ^T, Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75 ^T and P224 was 81.6% and 40.3% between strain T138021-75 ^T and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75 ^T belonged to a separate species that was closely related to strain P224. Strain P224 differed from strains T138021-75 ^T, Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for a-galactosidase and b-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75 ^T and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75 ^T (5CCUG 59995T5DSM 23207 ^T) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T _m).

Originalsprog	Engelsk
Tidsskrift	International Journal of Systematic and Evolutionary Microbiology
Vol/bind	61
Udgave nummer	7
Sider (fra-til)	1699-1704
Antal sider	6
ISSN	1466-5026
DOI	https://doi.org/10.1099/ijs.0.026518-0
Status	Udgivet - jul. 2011

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10.1099/ijs.0.026518-0

http://ijs.microbiologyresearch.org/deliver/fulltext/ijsem/61/7/1699_ijs026518.pdf?itemId=/content/journal/ijsem/10.1099/ijs.0.026518-0&mimeType=pdf&isFastTrackArticle=

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@article{18d48a0d52da45bcb2ba3866ac8007a3,

title = "Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs",

abstract = "Strains T138021-75 T, Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75 T and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6%. These two strains showed 97.8% sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3% similarity with the type strain of Mannheimia varigena, but,97% similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9%). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9% gene sequence similarity) compared with the three other strains (T138021-75 T, Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75 T and P224 was 81.6% and 40.3% between strain T138021-75 T and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75 T belonged to a separate species that was closely related to strain P224. Strain P224 differed from strains T138021-75 T, Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for a-galactosidase and b-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75 T and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75 T (5CCUG 59995T5DSM 23207 T) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T m).",

author = "Henrik Christensen and Bojesen, {Anders Miki} and Magne Bisgaard",

year = "2011",

month = jul,

doi = "10.1099/ijs.0.026518-0",

language = "English",

volume = "61",

pages = "1699--1704",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Society for General Microbiology",

number = "7",

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TY - JOUR

T1 - Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

AU - Christensen, Henrik

AU - Bojesen, Anders Miki

AU - Bisgaard, Magne

PY - 2011/7

Y1 - 2011/7

N2 - Strains T138021-75 T, Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75 T and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6%. These two strains showed 97.8% sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3% similarity with the type strain of Mannheimia varigena, but,97% similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9%). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9% gene sequence similarity) compared with the three other strains (T138021-75 T, Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75 T and P224 was 81.6% and 40.3% between strain T138021-75 T and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75 T belonged to a separate species that was closely related to strain P224. Strain P224 differed from strains T138021-75 T, Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for a-galactosidase and b-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75 T and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75 T (5CCUG 59995T5DSM 23207 T) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T m).

AB - Strains T138021-75 T, Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75 T and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6%. These two strains showed 97.8% sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3% similarity with the type strain of Mannheimia varigena, but,97% similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9%). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9% gene sequence similarity) compared with the three other strains (T138021-75 T, Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75 T and P224 was 81.6% and 40.3% between strain T138021-75 T and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75 T belonged to a separate species that was closely related to strain P224. Strain P224 differed from strains T138021-75 T, Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for a-galactosidase and b-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75 T and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75 T (5CCUG 59995T5DSM 23207 T) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T m).

U2 - 10.1099/ijs.0.026518-0

DO - 10.1099/ijs.0.026518-0

M3 - Journal article

SN - 1466-5026

VL - 61

SP - 1699

EP - 1704

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 7

ER -

Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

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