De novo genetic engineering of the camalexin biosynthetic pathway

Morten Emil Møldrup; Bo Salomonsen; Fernando Geu Flores; Carl Erik Olsen; Barbara Ann Halkier

doi:10.1016/j.jbiotec.2013.06.013

De novo genetic engineering of the camalexin biosynthetic pathway

Morten Emil Møldrup, Bo Salomonsen, Fernando Geu Flores, Carl Erik Olsen, Barbara Ann Halkier

17 Citationer (Scopus)

Abstract

Camalexin is a tryptophan-derived phytoalexin that is induced in the model plant Arabidopsis thaliana upon pathogen attack. Only few genes in the biosynthetic pathway of camalexin remain unidentified, however, investigation of candidate genes for these steps has proven particularly difficult partly because of redundancy in the genome of Arabidopsis. Here we describe metabolic engineering of the camalexin biosynthetic pathway in the transient Nicotiana benthamiana expression system. Camalexin accumulated in levels corresponding to what is seen in induced Arabidopsis thaliana. We have used this system to evaluate candidate genes suggested to be involved in the camalexin pathway. This has provided biochemical evidence for CYP71A12 conducting same reaction as CYP71A13 in the pathway. We discuss the prospects of using metabolic engineering of camalexin, both with respect to engineering plant defense and as a tool for screening yet unidentified candidate genes in the camalexin pathway.

Originalsprog	Engelsk
Tidsskrift	Journal of Biotechnology
Vol/bind	167
Udgave nummer	3
Sider (fra-til)	296-301
Antal sider	6
ISSN	0168-1656
DOI	https://doi.org/10.1016/j.jbiotec.2013.06.013
Status	Udgivet - 2013

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10.1016/j.jbiotec.2013.06.013

Citationsformater

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title = "De novo genetic engineering of the camalexin biosynthetic pathway",

abstract = "Camalexin is a tryptophan-derived phytoalexin that is induced in the model plant Arabidopsis thaliana upon pathogen attack. Only few genes in the biosynthetic pathway of camalexin remain unidentified, however, investigation of candidate genes for these steps has proven particularly difficult partly because of redundancy in the genome of Arabidopsis. Here we describe metabolic engineering of the camalexin biosynthetic pathway in the transient Nicotiana benthamiana expression system. Camalexin accumulated in levels corresponding to what is seen in induced Arabidopsis thaliana. We have used this system to evaluate candidate genes suggested to be involved in the camalexin pathway. This has provided biochemical evidence for CYP71A12 conducting same reaction as CYP71A13 in the pathway. We discuss the prospects of using metabolic engineering of camalexin, both with respect to engineering plant defense and as a tool for screening yet unidentified candidate genes in the camalexin pathway.",

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T1 - De novo genetic engineering of the camalexin biosynthetic pathway

AU - Møldrup, Morten Emil

AU - Salomonsen, Bo

AU - Geu Flores, Fernando

AU - Olsen, Carl Erik

AU - Halkier, Barbara Ann

PY - 2013

Y1 - 2013

N2 - Camalexin is a tryptophan-derived phytoalexin that is induced in the model plant Arabidopsis thaliana upon pathogen attack. Only few genes in the biosynthetic pathway of camalexin remain unidentified, however, investigation of candidate genes for these steps has proven particularly difficult partly because of redundancy in the genome of Arabidopsis. Here we describe metabolic engineering of the camalexin biosynthetic pathway in the transient Nicotiana benthamiana expression system. Camalexin accumulated in levels corresponding to what is seen in induced Arabidopsis thaliana. We have used this system to evaluate candidate genes suggested to be involved in the camalexin pathway. This has provided biochemical evidence for CYP71A12 conducting same reaction as CYP71A13 in the pathway. We discuss the prospects of using metabolic engineering of camalexin, both with respect to engineering plant defense and as a tool for screening yet unidentified candidate genes in the camalexin pathway.

AB - Camalexin is a tryptophan-derived phytoalexin that is induced in the model plant Arabidopsis thaliana upon pathogen attack. Only few genes in the biosynthetic pathway of camalexin remain unidentified, however, investigation of candidate genes for these steps has proven particularly difficult partly because of redundancy in the genome of Arabidopsis. Here we describe metabolic engineering of the camalexin biosynthetic pathway in the transient Nicotiana benthamiana expression system. Camalexin accumulated in levels corresponding to what is seen in induced Arabidopsis thaliana. We have used this system to evaluate candidate genes suggested to be involved in the camalexin pathway. This has provided biochemical evidence for CYP71A12 conducting same reaction as CYP71A13 in the pathway. We discuss the prospects of using metabolic engineering of camalexin, both with respect to engineering plant defense and as a tool for screening yet unidentified candidate genes in the camalexin pathway.

U2 - 10.1016/j.jbiotec.2013.06.013

DO - 10.1016/j.jbiotec.2013.06.013

M3 - Journal article

C2 - 23830903

SN - 0168-1656

VL - 167

SP - 296

EP - 301

JO - Journal of Biotechnology

JF - Journal of Biotechnology

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ER -

De novo genetic engineering of the camalexin biosynthetic pathway

Abstract

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