Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

Henrik Næsted; Agnethe Holm; Tom Jenkins; Henrik Bjørn Nielsen; Cassandra A. Harris; Michael H. Beale; Mathias Andersen; Alexandra Mant; Henrik Vibe Scheller; Bilal Camara; Ole Mattsson; John Mundy

doi:10.1242/jcs.01360

Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

Henrik Næsted, Agnethe Holm, Tom Jenkins, Henrik Bjørn Nielsen, Cassandra A. Harris, Michael H. Beale, Mathias Andersen, Alexandra Mant, Henrik Vibe Scheller, Bilal Camara, Ole Mattsson, John Mundy

Functional genomics

49 Citationer (Scopus)

Abstract

The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.

Originalsprog	Engelsk
Tidsskrift	Journal of Cell Science
Vol/bind	117
Udgave nummer	20
Sider (fra-til)	4807-18
Antal sider	12
ISSN	0021-9533
DOI	https://doi.org/10.1242/jcs.01360
Status	Udgivet - 2004

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10.1242/jcs.01360

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Næsted, H., Holm, A., Jenkins, T., Nielsen, H. B., Harris, C. A., Beale, M. H., Andersen, M., Mant, A., Scheller, H. V., Camara, B., Mattsson, O., & Mundy, J. (2004). Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development. Journal of Cell Science, 117(20), 4807-18. https://doi.org/10.1242/jcs.01360

@article{2b1ee200954111dd86a6000ea68e967b,

title = "Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development",

abstract = "The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.",

author = "Henrik N{\ae}sted and Agnethe Holm and Tom Jenkins and Nielsen, {Henrik Bj{\o}rn} and Harris, {Cassandra A.} and Beale, {Michael H.} and Mathias Andersen and Alexandra Mant and Scheller, {Henrik Vibe} and Bilal Camara and Ole Mattsson and John Mundy",

note = "Keywords: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Carrier Proteins; Chloroplasts; Gene Expression Regulation, Plant; Genes, Reporter; Genetic Complementation Test; Light; Molecular Sequence Data; Phenotype; Pigmentation; Pigments, Biological; Plant Leaves; Recombinant Fusion Proteins; Sequence Alignment; Two-Hybrid System Techniques; Zinc Fingers",

year = "2004",

doi = "10.1242/jcs.01360",

language = "English",

volume = "117",

pages = "4807--18",

journal = "Journal of Cell Science",

issn = "0021-9533",

publisher = "The/Company of Biologists Ltd.",

number = "20",

}

TY - JOUR

T1 - Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

AU - Næsted, Henrik

AU - Holm, Agnethe

AU - Jenkins, Tom

AU - Nielsen, Henrik Bjørn

AU - Harris, Cassandra A.

AU - Beale, Michael H.

AU - Andersen, Mathias

AU - Mant, Alexandra

AU - Scheller, Henrik Vibe

AU - Camara, Bilal

AU - Mattsson, Ole

AU - Mundy, John

N1 - Keywords: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Carrier Proteins; Chloroplasts; Gene Expression Regulation, Plant; Genes, Reporter; Genetic Complementation Test; Light; Molecular Sequence Data; Phenotype; Pigmentation; Pigments, Biological; Plant Leaves; Recombinant Fusion Proteins; Sequence Alignment; Two-Hybrid System Techniques; Zinc Fingers

PY - 2004

Y1 - 2004

N2 - The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.

AB - The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.

U2 - 10.1242/jcs.01360

DO - 10.1242/jcs.01360

M3 - Journal article

C2 - 15340011

SN - 0021-9533

VL - 117

SP - 4807

EP - 4818

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 20

ER -

Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

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