Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Verena Untiet; Lisa M Moeller; Ximena Ibarra-Soria; Gabriela Sánchez-Andrade; Miriam Stricker; Eva M Neuhaus; Darren W Logan; Thomas Gensch; Marc Spehr

doi:10.1093/chemse/bjw077

Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Verena Untiet, Lisa M Moeller, Ximena Ibarra-Soria, Gabriela Sánchez-Andrade, Miriam Stricker, Eva M Neuhaus, Darren W Logan, Thomas Gensch, Marc Spehr

5 Citationer (Scopus)

Abstract

In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.

Originalsprog	Engelsk
Tidsskrift	Chemical Senses
Vol/bind	41
Udgave nummer	8
Sider (fra-til)	669-676
Antal sider	8
ISSN	0379-864X
DOI	https://doi.org/10.1093/chemse/bjw077
Status	Udgivet - 1 okt. 2016
Udgivet eksternt	Ja

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10.1093/chemse/bjw077

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title = "Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons",

abstract = "In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.",

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author = "Verena Untiet and Moeller, {Lisa M} and Ximena Ibarra-Soria and Gabriela S{\'a}nchez-Andrade and Miriam Stricker and Neuhaus, {Eva M} and Logan, {Darren W} and Thomas Gensch and Marc Spehr",

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T1 - Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

AU - Untiet, Verena

AU - Moeller, Lisa M

AU - Ibarra-Soria, Ximena

AU - Sánchez-Andrade, Gabriela

AU - Stricker, Miriam

AU - Neuhaus, Eva M

AU - Logan, Darren W

AU - Gensch, Thomas

AU - Spehr, Marc

PY - 2016/10/1

Y1 - 2016/10/1

N2 - In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.

AB - In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.

KW - Animals

KW - Calcium/metabolism

KW - Chloride Channels/metabolism

KW - Chlorides/analysis

KW - Cytosol/chemistry

KW - Dendrites/chemistry

KW - Mice

KW - Sensory Receptor Cells/chemistry

KW - Vomeronasal Organ/chemistry

U2 - 10.1093/chemse/bjw077

DO - 10.1093/chemse/bjw077

M3 - Journal article

C2 - 27377750

SN - 0379-864X

VL - 41

SP - 669

EP - 676

JO - Chemical Senses

JF - Chemical Senses

IS - 8

ER -

Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Abstract

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