TY - JOUR
T1 - Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers
AU - Coman, Vasile
AU - Gustavsson, Tobias
AU - Finkelsteinas, Arnonas
AU - von Wachenfeldt, Claes
AU - Hägerhäll, Cecilia
AU - Gorton, Lo
PY - 2009/11/11
Y1 - 2009/11/11
N2 - The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.
AB - The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.
KW - Bacillus subtilis/cytology
KW - Electrochemical Techniques
KW - Electrodes
KW - Electron Transport Complex II/genetics
KW - Genetic Engineering
KW - Osmium
KW - Polymers
U2 - 10.1021/ja905442a
DO - 10.1021/ja905442a
M3 - Journal article
C2 - 19886699
SN - 0002-7863
VL - 131
SP - 16171
EP - 16176
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 44
ER -